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Index / Biolin / DNA Polymerases - For High-Fidelity Applications : VELOCITY DNA Polymerase /Product Detail : BIO-21099 DNA Polymerases - For High-Fidelity Applications : VELOCITY DNA Polymerase
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DNA Polymerases - For High-Fidelity Applications : VELOCITY DNA Polymerase

 Price: 451   EUR
512   USD
350   GBP

Product name : DNA Polymerases - For High-Fidelity Applications : VELOCITY DNA Polymerase

Catalog number : BIO-21099

Quantity: 500 Units

Availability: Yes

Supplier name : Biolin

Data sheet Data sheet : Ask more or other datasheet now !

More Details about

  • High-speed, high-fidelity DNA polymerase
  • High processivity
  • Fast amplification
  • Shorter PCR runs for longer templates
  • Robust, requires minimal optimization of the reaction

  • Cloning techniques where high-fidelity is desirable
  • Blunt-end cloning
  • Amplification of difficult templates
  • Mutagenesis


VELOCITY is a fast thermostable enzyme possessing 5´-3´ DNA polymerase and 3´-5´ proofreading exonuclease activities. VELOCITY provides high-fidelity with an error-rate of 4.4 x 10-7 combined with a high speed DNA synthesis as fast as 15s/Kb for templates of up to 5Kb and 30s/Kb for templates longer than 5Kb. The inherent high speed DNA synthesis is due to the enhanced processivity of the polymerase

VELOCITY is easy to use since it works with many different protocols and requires minimal optimization. The polymerase produces higher yields than most commercially available enzymes and generates blunt-ended amplicons. The error rate of VELOCITY DNA Polymerase (4.4 x 10-7) was determined using the rpsL fidelity assay (Lackovich et al., 2001; Fujii et al., 1999).

  • VELOCITY DNA Polymerase
  • 5x Hi-Fi Buffer (contains 10mM Mg2+)
  • 50mM MgCl2 Solution
  • DMSO

Working Concentration

One unit will incorporate 10nmoles of dNTPs in 30min at 72°C.

Storage Conditions

VELOCITY DNA Polymerase can be stored for 12 months at –20°C.
Shipping Conditions

On Dry Ice or Blue Ice.
Product Citations
  1. Killelea, T. & Connolly, B.A. Chem. BioChem. 12, 1330–1336 (2011).
  2. Thornton, J.K., et al. PLoS ONE 6(8), e23878 (2011).
  3. Paxton, C.W., et al. Am. J. Physiol. 300, C1345-C1355 (2011).
  4. Renaud, C., et al. J. Clin. Virol. 49(1), 21-25 (2010).
  5. Huang, X.X., et al. Am. J. Pathol. 174(4), 1534–1543 (2009).
  6. Norgate, M., et al. PLoS One. 4(11), e7950 (2009).

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