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Index / Biolin / DNA Polymerases - For Hot-Start Applications : MyTaq HS Red DNA Polymerase /Product Detail : BIO-21116 DNA Polymerases - For Hot-Start Applications : MyTaq HS Red DNA Polymerase
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DNA Polymerases - For Hot-Start Applications : MyTaq HS Red DNA Polymerase

 Price: 1066   EUR
1210   USD
827   GBP

Product name : DNA Polymerases - For Hot-Start Applications : MyTaq HS Red DNA Polymerase

Catalog number : BIO-21116

Quantity: 2500 units

Availability: Yes

Supplier name : Biolin

Data sheet Data sheet : Ask more or other datasheet now !

More Details about

 

"The MyTaq HS sample was so much better than anything else we have used, we have ordered more already."
 

Features

New generation of antibody-based hot-start polymerase

The highest specificity and the best performance

New buffer system including dNTPs and MgCl2

Fast PCR reactions

Red dye for loading the gel directly

 

Applications

High-throughput PCR

Assays with prolonged reaction setup on the bench or liquid handling

Amplification of challenging targets susceptible to mispriming

Colony PCR

Multiplexing

High Specificity of amplification of difficult templates ( such as GC rich)

Genotyping

TA cloning

Two-Step RT-PCR


Description

MyTaq™ HS Red DNA Polymerase is a high performance PCR reagent that is powered by antibody-mediated hot-start, specifically designed for fast, highly-specific, hot-start PCR. MyTaq HS Red does not possess polymerase activity during the reaction set-up, thus reducing non-specific amplification including primer-dimer formation. The advanced formulation of MyTaq HS Red allows fast cycling conditions, considerably reducing the reaction time without compromising PCR specificity or yield (Fig. 1). This new enzyme from Bioline is supplied with 5x MyTaq buffer system, a proprietary formulation that saves time and delivers superior results, as it contains dNTPs, MgCl2 and enhancers at optimal concentrations which removes the need for optimization.

The specially designed MyTaq Red formulation does not interfere with the PCR reaction and enables users to load samples directly onto a gel after the PCR without the need to add loading buffer.

MyTaq HS Red only requires the addition of template, primers and water, reducing the risk of pipetting errors and contamination as well as reducing the set-up time.


Components


•MyTaq HS DNA Polymerase
•5x MyTaq Reaction Buffer

 

Unit Definition

One unit is defined as the amount of enzyme that incorporates 10nmoles of dNTPs into acid insoluble form in 30 minutes at 72°C.


Concentration

5u/µl


Storage Conditions

MyTaq HS Red DNA Polymerase can be stored for up to 6 months at -20°C, or up to 2 weeks at +4°C. Repeated freeze/thaw cycles should be avoided.

 

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