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Index / Ray Biotech / Human_Mouse Phospho-Stat 4 (Y693) Cell-Based ELISA (1) ELISA /Product Detail : CBEL-STAT4-1 Human_Mouse Phospho-Stat 4 (Y693) Cell-Based ELISA (1) ELISA
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#CBEL-STAT4-1 Human_Mouse Phospho-Stat 4 (Y693) Cell-Based ELISA (1) ELISA

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  Price : 422   EUR
479   USD
327   GBP
1775   Zloty
56540   JPY
3258   NOK
3491   SEK
477   CHF

Product name : Human_Mouse Phospho-Stat 4 (Y693) Cell-Based ELISA (1) ELISA

Catalog number : CBEL-STAT4-1

Quantity: One 96-Well Microplate Kit

Availability: Yes

Supplier name : Ray Biotech

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About this Product :

Human_Mouse Phospho-Stat 4 (Y693) Cell-Based ELISA (1) ELISA
http://mybiofast.com/ver.php?search=ELISA&submit=Search
Storage and handling
All components of the Human_Mouse Phospho-Stat 4 (Y693) Cell-Based ELISA (1) ELISA should be stored at temperatures between 2 and 8 degrees Celsius. In order to keep the sensitivity of the kit, please don not freeze and thaw repeatedly.

Human_Mouse Phospho-Stat 4 (Y693) Cell-Based ELISA (1) ELISA mus musculus murine Human_Mouse Phospho-Stat 4 (Y693) Cell-Based ELISA (1) ELISA detects proteins from variouse species most likely human.
http://mybiofast.com/ver.php?search=musculus&submit=Search

More Details about

Product Features

  • Site and signal pathway-specific
  • In vitro detection of adherent cell culture
  • No sample lysis needed
  • Compatible with a standard ELISA plate reader
  • Faster results than with ELISA
  • Adaptable for high-throughput screening and drug discovery

Target Name/s

STAT4

Species Detected

Human, Mouse

Application Notes

Kit Components
  • Cell Culture Microplate
  • Wash Buffer A
  • Wash Buffer B
  • Fixing Solution
  • HRP-Conjugated Secondary Antibody
  • Blocking Buffer
  • TMB One-Step Substrate
  • Stop Solution
  • Quenching Buffer
Other Materials Required
  • Distilled or deionized water
  • 100 ml and 1 liter graduated cylinders
  • Tubes to prepare sample dilutions
  • Protease and Phosphatase inhibitors
  • Precision pipettes to deliver 2 µl to 1 ml volumes
  • Adjustable 1-25 ml pipettes for reagent preparation
  • Benchtop rocker or shaker
  • Microplate reader capable of measuring absorbance at 450 nm
Protocol Outline
  1. Seed 10,000-30,000 cells into each well and incubate overnight.
  2. Apply various treatment, inhibitors or activators according to manufacture's instructions.
  3. Add 100 µl of Fixing Solution into each well and incubate for 20 min at RT with shaking.
  4. Add 200 µl of prepared 1X Quenching Buffer and incubate 20 min at RT.
  5. Add 200 µl of Blocking Solution and incubate for 1 h at 37 °C.
  6. Add 50 µl of 1X anti-phospho-protein specific antibody or anti-pan-protein specific antibody to each well and incubate for 2 h at RT.
  7. Add 50 µl of prepared 1X HRP-Anti-Rabbit or Mouse IgG and incubate for 1 h at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Specificity

The antibody pair provided in this kit recognizes human and mouse Stat 4 (pTyr693).

Storage/Stability

-20°C
 
 
 
 

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY. Not for diagnostic or therapeutic use.

 

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Pathways :
WP1011: T Cell Receptor Signaling Pathway
WP1025: B Cell Receptor Signaling Pathway
WP1043: Calcium Regulation in the Cardiac Cell
WP1069: Integrin-mediated cell adhesion
WP1078: G1 to S cell cycle control
WP1083: Cell cycle
WP1130: T Cell Receptor Signaling Pathway
WP1144: B Cell Receptor Signaling Pathway
WP1159: Calcium Regulation in the Cardiac Cell
WP1185: Integrin-mediated cell adhesion
WP1195: G1 to S cell cycle control
WP1200: Cell cycle
WP1345: T Cell Receptor Signaling Pathway
WP1354: B Cell Receptor Signaling Pathway
WP1365: Calcium Regulation in the Cardiac Cell
WP1386: Integrin-mediated cell adhesion
WP1393: Cell cycle
WP1411: Cell Division: First embryonic mitosis
WP1775: Cell Cycle Checkpoints
WP1782: APC/C-mediated degradation of cell cycle proteins
WP179: Cell cycle
WP1792: Cell Cycle, Mitotic
WP1793: Cell junction organization
WP1794: Cell surface interactions at the vascular wall
WP1829: Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell

Related Genes :
[Stat1] Signal transducer and activator of transcription 1
[Pias4 Piasg] E3 SUMO-protein ligase PIAS4 (EC 2.3.2.27) (PIASy) (Protein inhibitor of activated STAT protein 4) (Protein inhibitor of activated STAT protein gamma) (PIAS-gamma) (RING-type E3 ubiquitin transferase PIAS4)
[PIAS4 PIASG] E3 SUMO-protein ligase PIAS4 (EC 2.3.2.27) (PIASy) (Protein inhibitor of activated STAT protein 4) (Protein inhibitor of activated STAT protein gamma) (PIAS-gamma) (RING-type E3 ubiquitin transferase PIAS4)
[Ceacam1 Bgp Bgp1] Carcinoembryonic antigen-related cell adhesion molecule 1 (Biliary glycoprotein 1) (BGP-1) (Biliary glycoprotein D) (MHVR1) (Murine hepatitis virus receptor) (MHV-R) (CD antigen CD66a)
[Stat4] Signal transducer and activator of transcription 4
[Erbb4 Mer4] Receptor tyrosine-protein kinase erbB-4 (EC 2.7.10.1) (Proto-oncogene-like protein c-ErbB-4) [Cleaved into: ERBB4 intracellular domain (4ICD) (E4ICD) (s80HER4)]
[STAT1] Signal transducer and activator of transcription 1-alpha/beta (Transcription factor ISGF-3 components p91/p84)
[Stat3 Aprf] Signal transducer and activator of transcription 3 (Acute-phase response factor)
[Pias1 Ddxbp1] E3 SUMO-protein ligase PIAS1 (EC 2.3.2.27) (DEAD/H box-binding protein 1) (Protein inhibitor of activated STAT protein 1) (RING-type E3 ubiquitin transferase PIAS1)
[Pias2 Miz1 Piasx] E3 SUMO-protein ligase PIAS2 (EC 2.3.2.27) (Androgen receptor-interacting protein 3) (ARIP3) (DAB2-interacting protein) (DIP) (Msx-interacting zinc finger protein) (Protein inhibitor of activated STAT x) (Protein inhibitor of activated STAT2) (RING-type E3 ubiquitin transferase PIAS2)
[CDK9 CDC2L4 TAK] Cyclin-dependent kinase 9 (EC 2.7.11.22) (EC 2.7.11.23) (C-2K) (Cell division cycle 2-like protein kinase 4) (Cell division protein kinase 9) (Serine/threonine-protein kinase PITALRE) (Tat-associated kinase complex catalytic subunit)
[STAT6] Signal transducer and activator of transcription 6 (IL-4 Stat)
[Stat6] Signal transducer and transcription activator 6
[ERBB4 HER4] Receptor tyrosine-protein kinase erbB-4 (EC 2.7.10.1) (Proto-oncogene-like protein c-ErbB-4) (Tyrosine kinase-type cell surface receptor HER4) (p180erbB4) [Cleaved into: ERBB4 intracellular domain (4ICD) (E4ICD) (s80HER4)]
[Stat5b] Signal transducer and activator of transcription 5B
[Eno1 Eno-1] Alpha-enolase (EC 4.2.1.11) (2-phospho-D-glycerate hydro-lyase) (Enolase 1) (Non-neural enolase) (NNE)
[Stat5a Mgf Mpf] Signal transducer and activator of transcription 5A (Mammary gland factor)
[Cdk5 Cdkn5 Crk6] Cyclin-dependent-like kinase 5 (EC 2.7.11.1) (CR6 protein kinase) (CRK6) (Cell division protein kinase 5) (Serine/threonine-protein kinase PSSALRE) (Tau protein kinase II catalytic subunit) (TPKII catalytic subunit)
[PIAS2 PIASX] E3 SUMO-protein ligase PIAS2 (EC 2.3.2.-) (Androgen receptor-interacting protein 3) (ARIP3) (DAB2-interacting protein) (DIP) (E3 SUMO-protein transferase PIAS2) (Msx-interacting zinc finger protein) (Miz1) (PIAS-NY protein) (Protein inhibitor of activated STAT x) (Protein inhibitor of activated STAT2)
[Mapk14 Crk1 Csbp1 Csbp2] Mitogen-activated protein kinase 14 (MAP kinase 14) (MAPK 14) (EC 2.7.11.24) (CRK1) (Mitogen-activated protein kinase p38 alpha) (MAP kinase p38 alpha)
[PTPN2 PTPT] Tyrosine-protein phosphatase non-receptor type 2 (EC 3.1.3.48) (T-cell protein-tyrosine phosphatase) (TCPTP)
[STAT5A STAT5] Signal transducer and activator of transcription 5A
[Kit Sl] Mast/stem cell growth factor receptor Kit (SCFR) (EC 2.7.10.1) (Proto-oncogene c-Kit) (Tyrosine-protein kinase Kit) (CD antigen CD117)
[Kitlg Kitl Mgf Sl Slf] Kit ligand (Hematopoietic growth factor KL) (Mast cell growth factor) (MGF) (Steel factor) (Stem cell factor) (SCF) (c-Kit ligand) [Cleaved into: Soluble KIT ligand (sKITLG)]
[Mapk1 Erk2 Mapk Prkm1] Mitogen-activated protein kinase 1 (MAP kinase 1) (MAPK 1) (EC 2.7.11.24) (ERT1) (Extracellular signal-regulated kinase 2) (ERK-2) (MAP kinase isoform p42) (p42-MAPK) (Mitogen-activated protein kinase 2) (MAP kinase 2) (MAPK 2)
[Cxcr4 Cmkar4 Lestr Sdf1r] C-X-C chemokine receptor type 4 (CXC-R4) (CXCR-4) (Fusin) (Leukocyte-derived seven transmembrane domain receptor) (LESTR) (Pre-B-cell-derived chemokine receptor) (PB-CKR) (Stromal cell-derived factor 1 receptor) (SDF-1 receptor) (CD antigen CD184)
[STAT3 APRF] Signal transducer and activator of transcription 3 (Acute-phase response factor)
[Cdk9] Cyclin-dependent kinase 9 (EC 2.7.11.22) (EC 2.7.11.23) (Cell division protein kinase 9)
[KIT SCFR] Mast/stem cell growth factor receptor Kit (SCFR) (EC 2.7.10.1) (Piebald trait protein) (PBT) (Proto-oncogene c-Kit) (Tyrosine-protein kinase Kit) (p145 c-kit) (v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog) (CD antigen CD117)
[Fgfr4 Fgfr-4 Mpk-11] Fibroblast growth factor receptor 4 (FGFR-4) (EC 2.7.10.1) (Protein-tyrosine kinase receptor MPK-11) (CD antigen CD334)

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