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Index / Ray Biotech / Human IFN-gamma ELISA (For Lysates) ELISA /Product Detail : ELH-IFNgamma-001C Human IFN-gamma ELISA (For Lysates) ELISA
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#ELH-IFNgamma-001C Human IFN-gamma ELISA (For Lysates) ELISA

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  Price : 440   EUR
500   USD
341   GBP
1851   Zloty
58973   JPY
3399   NOK
3642   SEK
498   CHF

Product name : Human IFN-gamma ELISA (For Lysates) ELISA

Catalog number : ELH-IFNgamma-001C

Quantity: One 96-Well Strip Microplate Kit

Availability: Yes

Supplier name : Ray Biotech

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About this Product :

Human IFN-gamma ELISA (For Lysates) ELISA
http://mybiofast.com/ver.php?search=eia&submit=Search
ELISA test expiration date
We recommend storage between 3 and 7 C. For shippment the temperature can be up to 25C during 2 days. This is mostly due to the stabilyty of the standard of the Human IFN-gamma ELISA (For Lysates) ELISA . Alternatively we can ship the standard on blue ice.

More Details about

Product Features

Sensitivity
15 pg/ml
Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Target Name/s

IFNgamma

Species Detected

Human

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Specificity

The antibody pair provided in this kit recognizes human IFN-gamma.

Storage/Stability

May be stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: the kit can be used within one year if the whole kit is stored at -20°C.
 
 
 
 

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY. Not for diagnostic or therapeutic use.

 

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Pathways :
WP1224: EBV LMP1 signaling
WP1225: estrogen signalling
WP152: FGF signaling pathway
WP1654: gamma-Hexachlorocyclohexane degradation
WP1722: Th1/Th2
WP1836: Interferon gamma signaling
WP1886: Post-translational modification: gamma carboxylation and hypusine formation
WP1993: angiogenesis overview
WP2006: Squamous cell TarBase
WP2107: pathways in human cancer
WP2152: BDNF
WP2199: Seed Development
WP2292: Chemokine signaling pathway
WP262: EBV LMP1 signaling
WP32: Translation Factors
WP537: Translation Factors
WP542: Electron Transport Chain
WP758: EBV LMP1 signaling
WP760: estrogen signalling
WP866: EBV LMP1 signaling
WP868: estrogen signalling

Related Genes :
[IFNGR1] Interferon gamma receptor 1 (IFN-gamma receptor 1) (IFN-gamma-R1) (CDw119) (Interferon gamma receptor alpha-chain) (IFN-gamma-R-alpha) (CD antigen CD119)
[IFNG] Interferon gamma (IFN-gamma) (Immune interferon)
[IL18 IGIF IL1F4] Interleukin-18 (IL-18) (Iboctadekin) (Interferon gamma-inducing factor) (IFN-gamma-inducing factor) (Interleukin-1 gamma) (IL-1 gamma)
[IRF9 ISGF3G] Interferon regulatory factor 9 (IRF-9) (IFN-alpha-responsive transcription factor subunit) (ISGF3 p48 subunit) (Interferon-stimulated gene factor 3 gamma) (ISGF-3 gamma) (Transcriptional regulator ISGF3 subunit gamma)
[IFNAR2 IFNABR IFNARB] Interferon alpha/beta receptor 2 (IFN-R-2) (IFN-alpha binding protein) (IFN-alpha/beta receptor 2) (Interferon alpha binding protein) (Type I interferon receptor 2)
[IFNLR1 IL28RA LICR2] Interferon lambda receptor 1 (IFN-lambda receptor 1) (IFN-lambda-R1) (Cytokine receptor class-II member 12) (Cytokine receptor family 2 member 12) (CRF2-12) (Interleukin-28 receptor subunit alpha) (IL-28 receptor subunit alpha) (IL-28R-alpha) (IL-28RA) (Likely interleukin or cytokine receptor 2) (LICR2)
[APP A4 AD1] Amyloid-beta precursor protein (APP) (ABPP) (APPI) (Alzheimer disease amyloid protein) (Amyloid precursor protein) (Amyloid-beta A4 protein) (Cerebral vascular amyloid peptide) (CVAP) (PreA4) (Protease nexin-II) (PN-II) [Cleaved into: N-APP; Soluble APP-alpha (S-APP-alpha); Soluble APP-beta (S-APP-beta); C99 (Beta-secretase C-terminal fragment) (Beta-CTF); Amyloid-beta protein 42 (Abeta42) (Beta-APP42); Amyloid-beta protein 40 (Abeta40) (Beta-APP40); C83 (Alpha-secretase C-terminal fragment) (Alpha-CTF); P3(42); P3(40); C80; Gamma-secretase C-terminal fragment 59 (Amyloid intracellular domain 59) (AICD-59) (AID(59)) (Gamma-CTF(59)); Gamma-secretase C-terminal fragment 57 (Amyloid intracellular domain 57) (AICD-57) (AID(57)) (Gamma-CTF(57)); Gamma-secretase C-terminal fragment 50 (Amyloid intracellular domain 50) (AICD-50) (AID(50)) (Gamma-CTF(50)); C31]
[IFNG] Interferon gamma (IFN-gamma)
[IFNG] Interferon gamma (IFN-gamma)
[IFNG] Interferon gamma (BoIFNG) (IFN-gamma)
[IFNG] Interferon gamma (IFN-gamma)
[IFNG] Interferon gamma (IFN-gamma)
[IFNG] Interferon gamma (IFN-gamma)
[IFNG] Interferon gamma (IFN-gamma)
[IFNG] Interferon gamma (IFN-gamma)
[IFNG] Interferon gamma (IFN-gamma)
[Ifng] Interferon gamma (IFN-gamma)
[IFNG] Interferon gamma (IFN-gamma)
[IFNG] Interferon gamma (IFN-gamma)
[IFNG] Interferon gamma (IFN-gamma)
[IFNG] Interferon gamma (IFN-gamma)
[IFNG] Interferon gamma (IFN-gamma)
[Ifng] Interferon gamma (IFN-gamma)
[IFNG] Interferon gamma (IFN-gamma)
[IFNL1 IL29 ZCYTO21] Interferon lambda-1 (IFN-lambda-1) (Cytokine Zcyto21) (Interleukin-29) (IL-29)
[Il18 Igif] Interleukin-18 (IL-18) (Interferon gamma-inducing factor) (IFN-gamma-inducing factor) (Interleukin-1 gamma) (IL-1 gamma)
[TICAM1 PRVTIRB TRIF] TIR domain-containing adapter molecule 1 (TICAM-1) (Proline-rich, vinculin and TIR domain-containing protein B) (Putative NF-kappa-B-activating protein 502H) (Toll-interleukin-1 receptor domain-containing adapter protein inducing interferon beta) (MyD88-3) (TIR domain-containing adapter protein inducing IFN-beta)
[IFNG] Interferon gamma (IFN-gamma)
[IFNK UNQ6124/PRO20084] Interferon kappa (IFN-kappa)
[RACK1 GNB2L1 HLC7 PIG21] Receptor of activated protein C kinase 1 (Cell proliferation-inducing gene 21 protein) (Guanine nucleotide-binding protein subunit beta-2-like 1) (Guanine nucleotide-binding protein subunit beta-like protein 12.3) (Human lung cancer oncogene 7 protein) (HLC-7) (Receptor for activated C kinase) (Small ribosomal subunit protein RACK1) [Cleaved into: Receptor of activated protein C kinase 1, N-terminally processed (Guanine nucleotide-binding protein subunit beta-2-like 1, N-terminally processed)]

Bibliography :
[30025490] Antitumor Effects of DC Vaccine With ALA-PDT-Induced Immunogenic Apoptotic Cells for Skin Squamous Cell Carcinoma in Mice.
[27852920] Thrombin-Induced Inflammation in Human Decidual Cells Is Not Affected By Heparin.
[26951511] Antigen-specific T cell response from dendritic cell vaccination using side population cell-associated antigens targets hepatocellular carcinoma.
[26100176] Propionibacterium acnes catalase induces increased Th1 immune response in sarcoidosis patients.
[24258974] Immunologic monitoring of cancer vaccine trials using the ELISPOT assay.
[23218956] Differential expression of proopiomelanocortin (POMC) transcriptional variants in human skin cells.
[22503372] Immune activation, viral gene product expression and neurotoxicity in the HIV-1 transgenic rat.
[22048768] Pilot clinical trial of type 1 dendritic cells loaded with autologous tumor lysates combined with GM-CSF, pegylated IFN, and cyclophosphamide for metastatic cancer patients.
[21806815] Role of epithelial integrin-linked kinase in promoting intestinal inflammation: effects on CCL2, fibronectin and the T cell repertoire.
[21646793] Human eosinophils produce and release a novel chemokine, CCL23, in vitro.