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Index / Ray Biotech / Porcine IFN-gamma ELISA ELISA /Product Detail : ELP-IFNgamma-001 Porcine IFN-gamma ELISA ELISA
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#ELP-IFNgamma-001 Porcine IFN-gamma ELISA ELISA

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  Price : 440   EUR
500   USD
341   GBP
1851   Zloty
58973   JPY
3399   NOK
3642   SEK
498   CHF

Product name : Porcine IFN-gamma ELISA ELISA

Catalog number : ELP-IFNgamma-001

Quantity: One 96-Well Strip Microplate Kit

Availability: Yes

Supplier name : Ray Biotech

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About this Product :

Porcine IFN-gamma ELISA ELISA
http://biotech-map.com/aa_search/table_sample.html?q=ELISA%20kits&filter=
Description
Porcine IFN-gamma ELISA ELISA is a high standard ELISA kit made with antibodies and plates of best quality as means for succesful acomplishment of your experiments and achievement of excellent and reliable results. For optimal conditions in every step of the protocol starting with the dilution of the samples, followed by incubation, blocking and washing, well designed buffers are included in the kit.

More Details about

Product Features

Sensitivity
500 pg/ml
Detection Range
0.5 ng/ml - 150 ng/ml
Recommended Dilution (serum & plasma)
2 fold
Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Specificity

 
Species Detected
Porcine

Standard Curves

ELISA Standard Curve

ELISA Standard Dilutions

Spiking & Recovery Results

Sample Type Average % Recovery Range (%)
Serum 106.7 95-114
Plasma 88.19 79-97
Cell culture media 86.23 77-94

Linearity Results

Sample Type Average % of Expected Range (%)
Serum 131.2 (1:2 dilution); 127.2 (1:4 dilution) 122-140 (1:2 dilution); 118-136 (1:4 dilution)
Plasma 128.5 (1:2 dilution); 74.93 (1:4 dilution) 118-139 (1:2 dilution); 67-82 (1:4 dilution)
Cell culture media 126.6 (1:2 dilution); 96.05 (1:4 dilution) 119-134 (1:2 dilution); 72-120 (1:4 dilution)

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

May be stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: the kit can be used within one year if the whole kit is stored at -20°C.
 
 
 
 

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY. Not for diagnostic or therapeutic use.

 

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Pathways :
WP1224: EBV LMP1 signaling
WP1225: estrogen signalling
WP152: FGF signaling pathway
WP1654: gamma-Hexachlorocyclohexane degradation
WP1722: Th1/Th2
WP1836: Interferon gamma signaling
WP1886: Post-translational modification: gamma carboxylation and hypusine formation
WP1993: angiogenesis overview
WP2006: Squamous cell TarBase
WP2152: BDNF
WP2199: Seed Development
WP2292: Chemokine signaling pathway
WP262: EBV LMP1 signaling
WP32: Translation Factors
WP537: Translation Factors
WP542: Electron Transport Chain
WP758: EBV LMP1 signaling
WP760: estrogen signalling
WP866: EBV LMP1 signaling
WP868: estrogen signalling

Related Genes :
[IFNGR1] Interferon gamma receptor 1 (IFN-gamma receptor 1) (IFN-gamma-R1) (CDw119) (Interferon gamma receptor alpha-chain) (IFN-gamma-R-alpha) (CD antigen CD119)
[IFNG] Interferon gamma (IFN-gamma)
[IFNG] Interferon gamma (IFN-gamma) (Immune interferon)
[IFNG] Interferon gamma (BoIFNG) (IFN-gamma)
[IFNG] Interferon gamma (IFN-gamma)
[IFNG] Interferon gamma (IFN-gamma)
[IFNG] Interferon gamma (IFN-gamma)
[Ifng] Interferon gamma (IFN-gamma)
[IL18 IGIF IL1F4] Interleukin-18 (IL-18) (Iboctadekin) (Interferon gamma-inducing factor) (IFN-gamma-inducing factor) (Interleukin-1 gamma) (IL-1 gamma)
[IFNG] Interferon gamma (IFN-gamma)
[IFNG] Interferon gamma (IFN-gamma)
[IRF9 ISGF3G] Interferon regulatory factor 9 (IRF-9) (IFN-alpha-responsive transcription factor subunit) (ISGF3 p48 subunit) (Interferon-stimulated gene factor 3 gamma) (ISGF-3 gamma) (Transcriptional regulator ISGF3 subunit gamma)
[IFNG] Interferon gamma (IFN-gamma)
[IFNG] Interferon gamma (IFN-gamma)
[IFNG] Interferon gamma (IFN-gamma)
[IFNG] Interferon gamma (IFN-gamma)
[IFNG] Interferon gamma (IFN-gamma)
[Ifng] Interferon gamma (IFN-gamma)
[IFNG] Interferon gamma (IFN-gamma)
[IFNG] Interferon gamma (IFN-gamma)
[IFNG] Interferon gamma (IFN-gamma)
[IFNG] Interferon gamma (IFN-gamma)
[Il18 Igif] Interleukin-18 (IL-18) (Interferon gamma-inducing factor) (IFN-gamma-inducing factor) (Interleukin-1 gamma) (IL-1 gamma)
[IFN-g IFNG] Interferon gamma (IFN-gamma)
[Il18 Igif] Interleukin-18 (IL-18) (Interferon gamma-inducing factor) (IFN-gamma-inducing factor) (Interleukin-1 gamma) (IL-1 gamma)
[IL18 IGIF] Interleukin-18 (IL-18) (Interferon gamma-inducing factor) (IFN-gamma-inducing factor) (Interleukin-1 gamma) (IL-1 gamma)
[IFNG] Interferon gamma (IFN-gamma)
[IFNG CK820_G0041509] Interferon gamma (IFN-gamma)
[IFN-gamma] Interferon gamma (IFN-gamma)
[IFNAR2 IFNABR IFNARB] Interferon alpha/beta receptor 2 (IFN-R-2) (IFN-alpha binding protein) (IFN-alpha/beta receptor 2) (Interferon alpha binding protein) (Type I interferon receptor 2)

Bibliography :
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