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Index / Ray Biotech / Human_Mouse Phospho-p38 alpha (T180_Y182) and Total p38 ELISA ELISA /Product Detail : PEL-P38-T180-002 Human_Mouse Phospho-p38 alpha (T180_Y182) and Total p38 ELISA ELISA
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Human_Mouse Phospho-p38 alpha (T180_Y182) and Total p38 ELISA ELISA

 Price: 572   EUR
649   USD
444   GBP

Product name : Human_Mouse Phospho-p38 alpha (T180_Y182) and Total p38 ELISA ELISA

Catalog number : PEL-P38-T180-002

Quantity: One 96-Well Strip Microplate Kit

Availability: Yes

Supplier name : Ray Biotech

Data sheet Data sheet : Ask more or other datasheet now !

About this Product :

Human_Mouse Phospho-p38 alpha (T180_Y182) and Total p38 ELISA ELISA
http://biotech-map.com/aa_search/table_sample.html?q=ELISA%20kit&filter=
Storage and handling
The storage of the Human_Mouse Phospho-p38 alpha (T180_Y182) and Total p38 ELISA ELISA has to be done at temperatures two-eight degrees Celsius. It is recommended to avoid 'freezing- thawing' cycles in manner to preserve the kit's sensitivity.

Human_Mouse Phospho-p38 alpha (T180_Y182) and Total p38 ELISA ELISA mus musculus murine Human_Mouse Phospho-p38 alpha (T180_Y182) and Total p38 ELISA ELISA detects proteins from variouse species most likely human.
http://mybiofast.com/ver.php?search=musculus&submit=Search

More Details about

 

Product Feature

  • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
  • Screen numerous different cell lysates without performing a Western Blot analysis
  • Minimal hands-on time, convenient, and non-radioactive material
  • High sensitivity
  • High quality
  • Cost-effective
  • Complete kit with pre-coated 96 well strip-plate

Target Name

P38-T180

Target Species

Human

Species Detected

Human, Mouse

Application Notes

Suggested Application
Quantitative Protein Detection; Establishing Normal Range
Kit Components
  • Pre-Coated 96-Well Strip Microplate
  • Wash Buffer
  • Anti-Pan Antibody
  • HRP-Conjugated Secondary Antibody
  • Assay Diluent
  • TMB One-Step Substrate
  • Stop Solution
  • Lysis Buffer
  • Positive Control Sample
Other Materials Required
  • Distilled or deionized water
  • 100 ml and 1 liter graduated cylinders
  • Tubes to prepare sample dilutions
  • Protease and Phosphatase inhibitors
  • Precision pipettes to deliver 2 μl to 1 ml volumes
  • Adjustable 1-25 ml pipettes for reagent preparation
  • Benchtop rocker or shaker
  • Microplate reader capable of measuring absorbance at 450 nm
Protocol Outline
  1. Prepare all reagents and samples as instructed in the manual.
  2. Add 100 μl of sample or positive control to each well. Incubate 2.5 h at RT or O/N at 4 °C.
  3. Add 100 μl of prepared primary antibody to each well. Incubate 1 h at RT.
  4. Add 100 μl of prepared 1X HRP-Streptavidin to each well. Incubate 1 h at RT.
  5. Add 100 μl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT.
  6. Add 50 μl of Stop Solution to each well. Read at 450 nm immediately.

Specificity

The antibody pair provided in this kit recognizes human and mouse Phospho-P38 alpha (pThr180/pTyr182) + pan P38.

Storage/Stability

Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
 
 
 
 

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY. Not for diagnostic or therapeutic use.

 

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