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Index / Ray Biotech / Custom Human E-Cadherin ELISA ELISA /Product Detail : ELH-ECadherin-001 Custom Human E-Cadherin ELISA ELISA
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#ELH-ECadherin-001 Custom Human E-Cadherin ELISA ELISA

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  Price : 660   EUR
749   USD
512   GBP
2772   Zloty
88317   JPY
5090   NOK
5454   SEK
746   CHF

Product name : Custom Human E-Cadherin ELISA ELISA

Catalog number : ELH-ECadherin-001

Quantity: One 96-Well Strip Microplate Kit

Availability: Yes

Supplier name : Ray Biotech

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About this Product :

Custom Human E-Cadherin ELISA ELISA
opis kitu
## product_name ## test ELISA, który jest wytwarzany przy uzyciu najwyższej jakości przeciwciał oraz najlepszych płytek, aby zapewnić Państwu doskonałe i powtarzalne wyniki w swojej pracy. Specyficznie zaprojektowane bufory zapewniają optymalne warunki na każdym etapie pracy.

Custom Human E-Cadherin ELISA ELISA Human samples 80 % of the research is conducted on human samples. Gentaur suppliers human normal cells, cell lines, RNA extracts and lots of antibodies and ELISA kits to Human proteins as well as Custom Human E-Cadherin ELISA ELISA .
http://mybiofast.com/ver.php?search=human+&submit=Search

More Details about

Product Features

  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Target Name/s

ECadherin

Species Detected

Human

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Specificity

The antibody pair provided in this kit recognizes human E-Cadherin.

Storage/Stability

May be stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: the kit can be used within one year if the whole kit is stored at -20°C.
 
 
 
 

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY. Not for diagnostic or therapeutic use.

 

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Pathways :
WP152: FGF signaling pathway
WP1983: Splicing factor NOVA regulated synpatic proteins
WP2107: pathways in human cancer
WP566: canonical wnt - zebrafish

Related Genes :
[CDH1 CDHE UVO] Cadherin-1 (CAM 120/80) (Epithelial cadherin) (E-cadherin) (Uvomorulin) (CD antigen CD324) [Cleaved into: E-Cad/CTF1; E-Cad/CTF2; E-Cad/CTF3]
[CDH13 CDHH] Cadherin-13 (Heart cadherin) (H-cadherin) (P105) (Truncated cadherin) (T-cad) (T-cadherin)
[Cdh1] Cadherin-1 (ARC-1) (Epithelial cadherin) (E-cadherin) (Uvomorulin) (CD antigen CD324) [Cleaved into: E-Cad/CTF1; E-Cad/CTF2; E-Cad/CTF3]
[CTNNA1] Catenin alpha-1 (Alpha E-catenin) (Cadherin-associated protein) (Renal carcinoma antigen NY-REN-13)
[CDH3 CDHP] Cadherin-3 (Placental cadherin) (P-cadherin)
[PCDH12 UNQ395/PRO731] Protocadherin-12 (Vascular cadherin-2) (Vascular endothelial cadherin-2) (VE-cad-2) (VE-cadherin-2) [Cleaved into: Protocadherin-12, secreted form]
[DCHS1 CDH19 CDH25 FIB1 KIAA1773 PCDH16] Protocadherin-16 (Cadherin-19) (Cadherin-25) (Fibroblast cadherin-1) (Protein dachsous homolog 1)
[CDH1] Cadherin-1 (Epithelial cadherin) (E-cadherin) (Uvomorulin) (CD antigen CD324) [Cleaved into: E-Cad/CTF1; E-Cad/CTF2; E-Cad/CTF3]
[CDH1] Cadherin-1 (Epithelial cadherin) (E-cadherin) (CD antigen CD324) [Cleaved into: E-Cad/CTF1; E-Cad/CTF2; E-Cad/CTF3]
[RET CDHF12 CDHR16 PTC RET51] Proto-oncogene tyrosine-protein kinase receptor Ret (EC 2.7.10.1) (Cadherin family member 12) (Proto-oncogene c-Ret) [Cleaved into: Soluble RET kinase fragment; Extracellular cell-membrane anchored RET cadherin 120 kDa fragment]
[Cdh1] Cadherin-1 (Epithelial cadherin) (E-cadherin) (Uvomorulin) (CD antigen CD324) [Cleaved into: E-Cad/CTF1; E-Cad/CTF2; E-Cad/CTF3]
[FAT1 CDHF7 FAT] Protocadherin Fat 1 (Cadherin family member 7) (Cadherin-related tumor suppressor homolog) (Protein fat homolog) [Cleaved into: Protocadherin Fat 1, nuclear form]
[CDH1] Cadherin-1 (Epithelial cadherin) (E-cadherin) (Uvomorulin) (CD antigen CD324) [Cleaved into: E-Cad/CTF1; E-Cad/CTF2; E-Cad/CTF3]
[CDH23 KIAA1774 KIAA1812 UNQ1894/PRO4340] Cadherin-23 (Otocadherin)
[FAT2 CDHF8 KIAA0811 MEGF1] Protocadherin Fat 2 (hFat2) (Cadherin family member 8) (Multiple epidermal growth factor-like domains protein 1) (Multiple EGF-like domains protein 1)
[SIPA1L1 E6TP1 KIAA0440] Signal-induced proliferation-associated 1-like protein 1 (SIPA1-like protein 1) (High-risk human papilloma viruses E6 oncoproteins targeted protein 1) (E6-targeted protein 1)
[CTNND1 KIAA0384] Catenin delta-1 (Cadherin-associated Src substrate) (CAS) (p120 catenin) (p120(ctn)) (p120(cas))
[PCDH1] Protocadherin-1 (Cadherin-like protein 1) (Protocadherin-42) (PC42)
[DSG1 CDHF4] Desmoglein-1 (Cadherin family member 4) (Desmosomal glycoprotein 1) (DG1) (DGI) (Pemphigus foliaceus antigen)
[TRIP13 PCH2] Pachytene checkpoint protein 2 homolog (Human papillomavirus type 16 E1 protein-binding protein) (16E1-BP) (HPV16 E1 protein-binding protein) (Thyroid hormone receptor interactor 13) (Thyroid receptor-interacting protein 13) (TR-interacting protein 13) (TRIP-13)
[OR1D2 OLFR1] Olfactory receptor 1D2 (Olfactory receptor 17-4) (OR17-4) (Olfactory receptor OR17-6) (Olfactory receptor-like protein HGMP07E)
[shg E-cadherin CG3722] DE-cadherin (Protein shotgun)
[CCNB1IP1 C14orf18 HEI10] E3 ubiquitin-protein ligase CCNB1IP1 (EC 2.3.2.27) (Cyclin-B1-interacting protein 1) (Human enhancer of invasion 10) (RING-type E3 ubiquitin transferase CCNB1IP1)
[MAEA EMP HLC10 PIG5] E3 ubiquitin-protein transferase MAEA (EC 2.3.2.27) (Cell proliferation-inducing gene 5 protein) (Erythroblast macrophage protein) (Human lung cancer oncogene 10 protein) (HLC-10) (Macrophage erythroblast attacher) (P44EMLP)
[RACK1 GNB2L1 HLC7 PIG21] Receptor of activated protein C kinase 1 (Cell proliferation-inducing gene 21 protein) (Guanine nucleotide-binding protein subunit beta-2-like 1) (Guanine nucleotide-binding protein subunit beta-like protein 12.3) (Human lung cancer oncogene 7 protein) (HLC-7) (Receptor for activated C kinase) (Small ribosomal subunit protein RACK1) [Cleaved into: Receptor of activated protein C kinase 1, N-terminally processed (Guanine nucleotide-binding protein subunit beta-2-like 1, N-terminally processed)]
[Ctnna1 Catna1] Catenin alpha-1 (102 kDa cadherin-associated protein) (Alpha E-catenin) (CAP102)
[OR1E1 OR1E5 OR1E6 OR1E9P] Olfactory receptor 1E1 (Olfactory receptor 13-66) (OR13-66) (Olfactory receptor 17-2/17-32) (OR17-2) (OR17-32) (Olfactory receptor 1E5) (Olfactory receptor 1E6) (Olfactory receptor 5-85) (OR5-85) (Olfactory receptor OR17-18) (Olfactory receptor-like protein HGMP07I)
[GLRX2 GRX2 CGI-133] Glutaredoxin-2, mitochondrial
[Cdh17] Cadherin-17 (BILL-cadherin) (Liver-intestine cadherin) (LI-cadherin) (P130)
[Cdh3 Cdhp] Cadherin-3 (Placental cadherin) (P-cadherin)

Bibliography :
[30744595] Cancer-associated fibroblasts induce epithelial-mesenchymal transition of bladder cancer cells through paracrine IL-6 signalling.
[30689555] Esculetin enhances the inhibitory effect of 5-Fluorouracil on the proliferation, migration and epithelial-mesenchymal transition of colorectal cancer.
[29774633] A simple and cost-efficient adherent culture platform for human gastric primary cells, as an in vitro model for Helicobacter pylori infection.
[29534204] Is 8% O2 more normoxic than 21% O2 for long-term in vitro cultures of human primary term cytotrophoblasts?
[29399801] The Potential Role of Bile Acids in Acquired Laryngotracheal Stenosis.
[29029005] Biopsy-derived Intestinal Epithelial Cell Cultures for Pathway-based Stratification of Patients With Inflammatory Bowel Disease.
[28672399] The Effect of a p38 Mitogen-Activated Protein Kinase Inhibitor on Cellular Senescence of Cultivated Human Corneal Endothelial Cells.
[28433430] Complementary effect of hydroquinone and retinoic acid on corneocyte desquamation with their combination use.
[30226722] Pemphigus Vulgaris Presenting as Chronic Vulvovaginal Erosions: The Importance of Autoantibody Testing: A Case Report.
[27750286] Secretion Profile of Induced Pluripotent Stem Cell-Derived Retinal Pigment Epithelium During Wound Healing.