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Index / Ray Biotech / Custom Human EDA-A2 ELISA ELISA /Product Detail : ELH-EDAA2-001 Custom Human EDA-A2 ELISA ELISA
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#ELH-EDAA2-001 Custom Human EDA-A2 ELISA ELISA

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  Price : 660   EUR
749   USD
512   GBP
2772   Zloty
88317   JPY
5090   NOK
5454   SEK
746   CHF

Product name : Custom Human EDA-A2 ELISA ELISA

Catalog number : ELH-EDAA2-001

Quantity: One 96-Well Strip Microplate Kit

Availability: Yes

Supplier name : Ray Biotech

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About this Product :

Custom Human EDA-A2 ELISA ELISA
opis kitu
## PRODUCT_NAME ## Warunki przechowywania: Zalecamy aby przechowywyanie odbywało się w temperaturze od 2 do 8 stopni Celsjusza. Zdecydowanie należy unikać zamrażania i rozmrażania produktu aby kit zachował swoją czułośc detekcji.

Custom Human EDA-A2 ELISA ELISA Human samples 80 % of the research is conducted on human samples. Gentaur suppliers human normal cells, cell lines, RNA extracts and lots of antibodies and ELISA kits to Human proteins as well as Custom Human EDA-A2 ELISA ELISA .
http://mybiofast.com/ver.php?search=human+&submit=Search

More Details about

Product Features

  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Target Name/s

EDAA2

Species Detected

Human

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Specificity

The antibody pair provided in this kit recognizes human EDA-A2.

Storage/Stability

May be stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: the kit can be used within one year if the whole kit is stored at -20°C.
 
 
 
 

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY. Not for diagnostic or therapeutic use.

 

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