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Index / Ray Biotech / Custom Mouse TWEAK R ELISA ELISA /Product Detail : ELM-TWEAKR-001 Custom Mouse TWEAK R ELISA ELISA
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#ELM-TWEAKR-001 Custom Mouse TWEAK R ELISA ELISA

 Price: 660   EUR
749   USD
512   GBP

Product name : Custom Mouse TWEAK R ELISA ELISA

Catalog number : ELM-TWEAKR-001

Quantity: One 96-Well Strip Microplate Kit

Availability: Yes

Supplier name : Ray Biotech

Data sheet Data sheet : Ask more or other datasheet now !

About this Product :

Custom Mouse TWEAK R ELISA ELISA
http://gentaurshop.com/search?search=elisa
Advisory
During handling and transportation of the Custom Mouse TWEAK R ELISA ELISA some of the liquid components are possible to stick on the walls and to the lids of the vials. Therefore, for collection of the liquids from the cap we strongly advise you to centrifuge the vials for a short period.

Custom Mouse TWEAK R ELISA ELISA mus musculus murine Custom Mouse TWEAK R ELISA ELISA detects proteins from variouse species most likely human.
http://mybiofast.com/ver.php?search=musculus&submit=Search

More Details about

Product Features

Sensitivity
95 pg/ml
Detection Range
95 pg/ml - 25000 pg/ml
Recommended Dilution (serum & plasma)
3-30 fold
Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Specificity

This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- ?, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 ?, IL-1?, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP- 5, M-CSF, MIG, MIP-1?, MIP-1?, MIP-2, MIP-3?, MIP-3?, PF-4, PSelectin, RANTES, SCF, SDF-1?, TARC, TCA-3, TECK, TIMP-1, TNF-?, TNF RI, TNF RII, TPO, Tweak, VCAM-1, VEGF.
Species Detected
Mouse

Standard Curves

ELISA Standard Curve

ELISA Standard Dilutions

Spiking & Recovery Results

Sample Type Average % Recovery Range (%)
Serum 116 106-128
Plasma 121 108-135
Cell culture media 105.9 98-114

Linearity Results

Sample Type Average % of Expected Range (%)
Serum 109.5 (1:2 dilution); 127.3 (1:4 dilution) 102-118 (1:2 dilution); 119-135 (1:4 dilution)
Plasma 112.9 (1:2 dilution); 106.9 (1:4 dilution) 105-121 (1:2 dilution); 98-116 (1:4 dilution)
Cell culture media 105.2 (1:2 dilution); 108.8 (1:4 dilution) 97-113 (1:2 dilution); 103-114 (1:4 dilution)

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

May be stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: the kit can be used within one year if the whole kit is stored at -20°C.
 
 
 
 

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY. Not for diagnostic or therapeutic use.

 

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