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Index / Ray Biotech / Custom Rat Jagged 1 ELISA ELISA /Product Detail : ELR-Jagged1-001 Custom Rat Jagged 1 ELISA ELISA
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#ELR-Jagged1-001 Custom Rat Jagged 1 ELISA ELISA

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  Price : 660   EUR
749   USD
512   GBP
2772   Zloty
88317   JPY
5090   NOK
5454   SEK
746   CHF

Product name : Custom Rat Jagged 1 ELISA ELISA

Catalog number : ELR-Jagged1-001

Quantity: One 96-Well Strip Microplate Kit

Availability: Yes

Supplier name : Ray Biotech

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About this Product :

Custom Rat Jagged 1 ELISA ELISA
http://antibody-antibodies.com/anti-elisa/ELISA.html
EIA kit description
Custom Rat Jagged 1 ELISA ELISA is an ELISA kit which is manufactured by highest quality antibodies and plates to provide you with excellent and reproducible results in your work. The specifically designed buffers will ensure optimal conditions in each step from dilution of the samples, through the incubation to the blocking and washing.

More Details about

Product Features

  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Target Name/s

Jagged1

Species Detected

Rat

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Specificity

The antibody pair provided in this kit recognizes rat Jagged 1.

Storage/Stability

May be stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: the kit can be used within one year if the whole kit is stored at -20°C.
 
 
 
 

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY. Not for diagnostic or therapeutic use.

 

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Pathways :
WP566: canonical wnt - zebrafish

Related Genes :
[JAG1 JAGL1] Protein jagged-1 (Jagged1) (hJ1) (CD antigen CD339)
[Cacna1c Cach2 Cacn2 Cacnl1a1 Cchl1a1] Voltage-dependent L-type calcium channel subunit alpha-1C (Calcium channel, L type, alpha-1 polypeptide, isoform 1, cardiac muscle) (Rat brain class C) (RBC) (Voltage-gated calcium channel subunit alpha Cav1.2)
[Cacna1a Cach4 Cacn3 Cacnl1a4] Voltage-dependent P/Q-type calcium channel subunit alpha-1A (Brain calcium channel I) (BI) (Calcium channel, L type, alpha-1 polypeptide, isoform 4) (Rat brain class A) (RBA-I) (Voltage-gated calcium channel subunit alpha Cav2.1)
[Cacna1d Cach3 Cacn4 Cacnl1a2 Cchl1a2] Voltage-dependent L-type calcium channel subunit alpha-1D (Calcium channel, L type, alpha-1 polypeptide, isoform 2) (Rat brain class D) (RBD) (Voltage-gated calcium channel subunit alpha Cav1.3)
[Apex1 Ape Apex Ref1] DNA-(apurinic or apyrimidinic site) lyase (EC 3.1.-.-) (EC 4.2.99.18) (APEX nuclease) (APEN) (Apurinic-apyrimidinic endonuclease 1) (AP endonuclease 1) (REF-1) (Redox factor-1) [Cleaved into: DNA-(apurinic or apyrimidinic site) lyase, mitochondrial]
[Fut2 Ftb Sec1] Galactoside 2-alpha-L-fucosyltransferase 2 (EC 2.4.1.344) (EC 2.4.1.69) (Alpha 1,2-fucosyltransferase) (Alpha 1,2-fucosyltransferase B) (Alpha 1-2 fucosyltransferase) (Alpha(1,2)FT 2) (Fucosyltransferase 2) (GDP-L-fucose:beta-D-galactoside 2-alpha-L-fucosyltransferase 2) (Secretor blood group alpha-2-fucosyltransferase)
[Notch1] Neurogenic locus notch homolog protein 1 (Notch 1) [Cleaved into: Notch 1 extracellular truncation (NEXT); Notch 1 intracellular domain (NICD)]
[Oprm1 Ror-b] Mu-type opioid receptor (M-OR-1) (MOR-1) (Opioid receptor B)
[Ogg1 Mmh Ogh1] N-glycosylase/DNA lyase [Includes: 8-oxoguanine DNA glycosylase (EC 3.2.2.-); DNA-(apurinic or apyrimidinic site) lyase (AP lyase) (EC 4.2.99.18)]
[Ggta1 Ggta-1] N-acetyllactosaminide alpha-1,3-galactosyltransferase (EC 2.4.1.87) (UDP-galactose:beta-D-galactosyl-1,4-N-acetyl-D-glucosaminide alpha-1,3-galactosyltransferase) (Galactosyltransferase)
[Cyb5r4 Ncb5or] Cytochrome b5 reductase 4 (EC 1.6.2.2) (Flavohemoprotein b5/b5R) (b5+b5R) (N-terminal cytochrome b5 and cytochrome b5 oxidoreductase domain-containing protein) (cb5/cb5R)
[Abo Abo1] Histo-blood group ABO system transferase 1 (Blood group A glycosyltransferase 1) (Cis-AB transferase 1) (Fucosylglycoprotein 3-alpha-galactosyltransferase) (Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase) (Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase) (EC 2.4.1.40) (Glycoprotein-fucosylgalactoside alpha-galactosyltransferase) (EC 2.4.1.37) (Histo-blood group A transferase) (A transferase) (Histo-blood group B transferase) (B transferase) (N-acetylgalactosaminyltransferase A blood group-like enzyme) (NAGAT 1)
[Nr1h2 Lxrb] Oxysterols receptor LXR-beta (Liver X receptor beta) (Nuclear receptor subfamily 1 group H member 2) (Orphan nuclear receptor OR-1) (Ubiquitously-expressed nuclear receptor) (UR)
[Impa1 Imp] Inositol monophosphatase 1 (IMP 1) (IMPase 1) (EC 3.1.3.25) (D-galactose 1-phosphate phosphatase) (EC 3.1.3.94) (Inositol-1(or 4)-monophosphatase 1) (Lithium-sensitive myo-inositol monophosphatase A1)
[Abo2] Histo-blood group ABO system transferase 2 (B blood group galactosyltransferase) (Blood group A glycosyltransferase 2) (Cis-AB transferase 2) (Fucosylglycoprotein 3-alpha-galactosyltransferase) (Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase) (Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase) (EC 2.4.1.40) (Glycoprotein-fucosylgalactoside alpha-galactosyltransferase) (EC 2.4.1.37) (Histo-blood group A transferase) (A transferase) (Histo-blood group B transferase) (B transferase) (NAGAT 2) (Putative blood group A transferase T2)
[Notch2] Neurogenic locus notch homolog protein 2 (Notch 2) [Cleaved into: Notch 2 extracellular truncation; Notch 2 intracellular domain]
[Oprk1 Ror-d] Kappa-type opioid receptor (K-OR-1) (KOR-1)
[A3galt2] Alpha-1,3-galactosyltransferase 2 (EC 2.4.1.87) (Isoglobotriaosylceramide synthase) (iGb3 synthase) (iGb3S)
[Oprd1 Ror-a] Delta-type opioid receptor (D-OR-1) (DOR-1) (Opioid receptor A)
[Serpinf1 Dmrs91 rCG_34442] Alpha-2 antiplasmin (Serine (Or cysteine) peptidase inhibitor, clade F, member 1) (Serine (Or cysteine) peptidase inhibitor, clade F, member 1, isoform CRA_c) (Serpin family F member 1)
[Impad1 Impa3] Inositol monophosphatase 3 (IMP 3) (IMPase 3) (EC 3.1.3.25) (EC 3.1.3.7) (Golgi 3-prime phosphoadenosine 5-prime phosphate 3-prime phosphatase) (Golgi-resident PAP phosphatase) (gPAPP) (Inositol monophosphatase domain-containing protein 1) (Inositol-1(or 4)-monophosphatase 3) (Myo-inositol monophosphatase A3)
[Pofut1 Fut12] GDP-fucose protein O-fucosyltransferase 1 (EC 2.4.1.221) (Peptide-O-fucosyltransferase 1) (O-FucT-1)
[Tmprss11d Rat] Transmembrane protease serine 11D (EC 3.4.21.-) (Adrenal secretory serine protease) (AsP) (Airway trypsin-like protease) (AT) [Cleaved into: Transmembrane protease serine 11D non-catalytic chain; Transmembrane protease serine 11D catalytic chain]
[B4galt1 Ggtb2] Beta-1,4-galactosyltransferase 1 (Beta-1,4-GalTase 1) (Beta4Gal-T1) (b4Gal-T1) (EC 2.4.1.-) (Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase) (Beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase) (EC 2.4.1.38) (Lactose synthase A protein) (EC 2.4.1.22) (N-acetyllactosamine synthase) (EC 2.4.1.90) (Nal synthase) (UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 1) (UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1) (Fragments)
[Hspa1b Hsp70-2 Hspa2] Heat shock 70 kDa protein 1B (Heat shock 70 kDa protein 1) (HSP70-1) (HSP70.1)
[Gcnt3] Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3 (EC 2.4.1.102) (EC 2.4.1.148) (EC 2.4.1.150) (C2GnT-mucin type) (C2GnT-M) (dI/C2/C4GnT) (dIGnT)
[Hspa1a Hsp70-1 Hspa1] Heat shock 70 kDa protein 1A (Heat shock 70 kDa protein 2) (HSP70-2) (HSP70.2)
[Olr1743 9430032L10Rik Gabbr1 Mog Olr1735_predicted Olr1736_predicted Olr1737_predicted Olr1738_predicted Olr1739_predicted Olr1742_predicted Olr1743_predicted Olr1744_predicted Olr1745_predicted Olr1746_predicted Olr1748_predicted Olr1749_predicted Olr1750_predicted Or8 Rps15-ps RT1-M3 RT1-M3-2 RT1-M6-1 Ubd Zfp57 Znrd1] Olfactory receptor 1743 (Olfactory receptor 8, rat orthologue of mouse MOR121-1)
[Ces1d Ces3] Carboxylesterase 1D (Carboxyesterase ES-10) (Carboxylesterase 3) (EC 3.1.1.1) (EC 3.1.1.67) (ES-HVEL) (Fatty acid ethyl ester synthase) (FAEE synthase) (Liver carboxylesterase 10) (pI 6.1 esterase)
[Hsd3b1] 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1 (3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I) (3-beta-HSD I) (3-beta-hydroxy-5-ene steroid dehydrogenase) (3-beta-hydroxy-Delta(5)-steroid dehydrogenase) (EC 1.1.1.145) (3-beta-hydroxysteroid 3-dehydrogenase) (EC 1.1.1.270) (Delta-5-3-ketosteroid isomerase) (Dihydrotestosterone oxidoreductase) (EC 1.1.1.210) (Steroid Delta-isomerase) (EC 5.3.3.1)

Bibliography :
[24211762] Interactions between lens epithelial and fiber cells reveal an intrinsic self-assembly mechanism.