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Index / Bioner / ExiProgen /Product Detail : A-5041 ExiProgen
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ExiProgen

 Price: 19150   EUR
21735   USD
14855   GBP

Product name : ExiProgen

Catalog number : A-5041

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Availability: Yes

Supplier name : Bioner

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More Details about


Bioneer's ExiProgen™ is a fully automated system for various scientific purposes in synthetic biology including synthesis of functional proteins or simple enzymes, biosimilars, and even therapeutic antibodies. ExiProgen™ is able to synthesize and purify up to 16 proteins in a single run. Simply, plasmid DNA or linear PCR product is added to the system followed by the synthesis of protein from Bioneer's E. coli extract for the transcription and translation processes. Target proteins are further purified by Ni-NTA magnetic beads in the system using His-tag on the protein. ExiProgen™ yields up to 100ug of protein with the purity of >90% in approximately 6hrs. This versatile system also allows purification of nucleic acids from various sources including tissue, blood, bacteria, and plant. We believe that ExiProgen™ system will offer strong solutions for your research including drug discoveries.







Features and Benefits

figure1 Built-in protocols optimized for protein synthesis/purification and the extraction of a wide variety of Nucleic Acid Samples
ExiProgen™ has more than 900 built-in protocols. Each has been optimized for conditions from sample sources to target nucleic acids leading to a successful protein synthesis/purification. This user-friendly interface allows reproducible and reliable results. The instrument software is also able to be upgraded automatically through the network connection to maintain the system to up-to-date with the best performing protocols.





figure2 Cooling Unit
ExiProgen™ ensures sample integrity which allows target protein to maintain functionality by housing a cooling unit where the elution tube rack is located. This presents a solution for overnight running and provide confidence in your results.






figure3 Magnetic block & Heating Unit
ExiProgen™ provides a Magnet & Heating block for protein or nucleic acid purification, which allows better extraction efficiency. Magnetic field is applied for pulling magnetic particles from sample wells for washing and eluting proteins or nucleic acids. Temperature of the heating block is tightly controlled, allowing it to generate reliable and reproducible results for protein synthesis. This also allows for evaporating away any residual ethanol after nucleic acid purification (Patent KR 10-1025135).







figure4 Contamination Shield
Contamination shield is designed to protect the assay from cross-contamination during instrument operation. Whenever the pipette tips are moving, the contamination shield is always co-located under the tips, therefore eliminating the possibility of cross-contamination which is necessary when working with multiple samples.







figure4 Easy to use LCD Touch screen
The 3.5" touch screen maximizes efficiency by offering an intuitive interface with simple push-button operation for processes such as selecting protocols and controlling the UV sterilization lamp.







figure3 UV lamp
ExiProgen™ is equipped with a powerful UV sterilization lamp that enables the user to sterilize the instrument chamber before and/or after experiment. This removes possible inter-assay cross-contamination that may occur.







Experimental Procedure

figure6

Principle of protein synthesis and purification

ExiProgen™ Protein Synthesis Kit utilizes E. coli extract to effectively control the transcription/translation process from nucleic acid templates which can be plasmids, or PCR products. Target protein is generated with a His-Tag, which is then purified using the Ni-NTA magnetic bead provided. The final yield of purified protein is more than 100ug with the purity of over 90%.   

figure7


Nucleic acid extraction principle

ExiProgen™ DNA/ RNA Kits allows to extract nucleic acids efficiently through Bioneer's silica magnetic beads. High yields of ultrapure DNA or RNA are obtained with OD260/280 readings of > 1.8 for DNA and 2.0 for RNA. 

figure8

Function

Feature Benefit
Fast Protein production From DNA to Pure protein in about 6 hours
DNA purification A wide variety of gDNA kits available
RNA purification A wide variety of RNA kits available
Affinity protein purification For couples expression and purification in one system!


 

Applications

Enzyme engineering, protein evolution, Synthetic biology, Bio-energy R&D, Protein drug R&D, expression screening, functional analysis, assay development and antigen production.

 

Specifications

   Dimensions (W X H X D) 320 mm x 500 mm x 535 mm
   Weight 27 kg
   Temperature range 15 - 30°C
   Humidity range 20 - 80%, no condensation
   Operating system Built-in
   User interface 320 x 240 touch screen graphic LCD
   Input Voltage 100 - 240 VAC
   Frequency 50 / 60 Hz
   UV sterilization 5-minute cycle, 48.96 mJ/cm energy max 2
   Communications TCP/IP
   Heat block 40 - 95°C


 

Functions

  ExiProgen
DNA/RNA Extraction Yes
Protein Expression Yes
Protein Purification Yes
TFT Touch Screen Yes
Sample Number 16 samples
UV sterilization Yes
Heated magnetic block Yes
Contamination shield Yes
TCP/IP network connection Multiple systems can be daisy-chained together (up to 6)
Cooling system for sample storeage Yes
Automatic dispensing Yes
Power supply 24 VDC, 7.5 A(180 W)


 

Experimental Data

1. Protein expression and purification
- Amount of input DNA: 6 - 10 ug (purity >1.8)
- Protocol number : 902
- Total prep time : < 6 h
- Yield: ~100 ug

 

Procedure

 

SDS-PAGE analysis


Figure 1. GFP protein (His-tagged protein)’s expression/purification with ExiProgen™.
M; AccuLadder™ Protein Size Maker (Low)
E; expression sample
P; purification sample
 

Figure 2. Various His-tagged proteins expressed/purified withExiProgen™.
M; AccuLadder™ Protein Size Maker (Low)
E; expression sample
P; purification sample

 



 

2. Total RNA Extraction from Tissue or cultured mammalian cell
- Sample: 15 ㎎ (Rat liver tissue) or 1 X 106 cells (HeLa)
- Protocol number: 202
- Average yield: 25 ug (Rat liver tissue), 10 ug (HeLa)
- Average RQS: > 9.5 

RQS(RNA quality score): The Caliper RQS is a calculated score that rates the quality of RNA samples. The RQS has been validated to correlate well with Agilent’s RIN (RNA Integrity Number) and follows the same 0-10 scale rating. Results comparing RIN to RQS for the same samples run on both LabChip GX and Agilent's Bioanalyzer 2100 typically show <10% deviation ( http://www.caliperls.jp/assets/pdf/lcgx/lcgx-AP-402.pdf)

 

Procedure 

 

Rat total RNA Extracted from tissue


Total RNA from Rat was observed to contain intact rRNA with no evidence of degradation.


 

Real-Time PCR carried out on Rat Total RNA

Real time RT-PCR was carried out.AccuPower® RocketScrpt™ RT PreMix(K-2101, Bioneer), and AccuPower® DualStar™ qPCR PreMix(K-6110, Bioneer) , and the Exicycler™ 96 Real-Time PCR system (A-2060).


 

Analysis of total RNA from HeLa cells. 

Total RNA was extracted from HeLa cell (1 X 106 cells) and analyzed using the LabChip GX (Caliper Life Science).
Each subunit of rRNA is clearly seen with no detectable degradation . In addition, the average yield of Total RNA was 10 ug, and the average RQS was 9.5 or higher.


 

Real-Time PCR carried out on total RNA from Hela cells. 

Real time RT-PCR was carried out on total RNA extracted from HeLa cell (1 X 106 cells) using AccuPower® RocketScrpt™ RT PreMix(K-2101, Bioneer), AccuPower® DualStar™ qPCR PreMix(K-6110, Bioneer) and the Exicycler™ 96 Real-Time PCR system(A-2060)


 

3. Genomic DNA from Cultured mammalian cell (HeLa))
- Sample volume: 1 X 106 cells
- Protocol number: 109
- Total prep time: < 1hr
- Average yield: 4-8 ug

 

Procedure

 

Agarose gel analysis

Lanes 1, 3, 5, 7, 9, 11, 13, 15 were extracted with 1X106 cells of cultured HeLa cell and Lanes 2, 4, 6, 8, 10, 12, 14, 16 were extracted with ddH2O as a negative control in DNA extraction. Note all samples have very similar yields.
Purity was also tested and was consistently between 1.8 and 2.0 (not shown).


 

4. Genomic DNA from Bacteria (E.coli)
- Sample volume: 1 X 106 cells
- Protocol number: 109
- Total prep time: < 1hr
- Average yield: 4-8 ug

 

Procedure

 

Agarose gel analysis

Lanes 1, 3, 5, 7, 9, 11, 13, 15 were extracted with 1X106 cells of E.coli cells and Lanes 2, 4, 6, 8, 10, 12, 14, 16 were extracted with ddH2O as a negative control in DNA extraction. Note all samples have very similar yields.
Purity was also tested and was consistently between 1.9 and 2.0 (not shown).


 

5. Genomic DNA from Tissue (Bovine skeletal muscle)
- Sample volume: 1 X 106 cells
- Protocol number: 109
- Total prep time: < 1hr
- Average yield: 8-12 ug

 

Procedure

 

Agarose gel analysis

Lanes 1, 3, 5, 7, 9, 11, 13, 15 were extracted with 20 mg of bovine tissues and Lanes 2, 4, 6, 8, 10, 12, 14, 16 were extracted with ddH2O as a negative control in DNA extraction. Note all samples have very similar yields.
Purity was also tested and was consistently between 1.9 and 2.0 (not shown).


 

6. Genomic DNA from Plant Tissue (Spinach leaf)
- Sample volume: 100 ㎎
- Protocol number: 104
- Total prep time: <1 hr
- Average yield: 3-5 ug

 

Procedure

 

Agarose gel analysis

Lanes 1, 3, 5, 7, 9, 11, 13, 15 were extracted with 100 mg of spinach leaves and Lanes 2, 4, 6, 8, 10, 12, 14, 16 were extracted with ddH2O as a negative control in DNA extraction. Note all samples have very similar yields.
Purity was also tested and was consistently between 1.9 and 2.0 (not shown).

 

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