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rHuman Activin A Active, Human cytokinesand growth factors
/Product Detail : RF0010-10 rHuman Activin A Active, Human cytokinesand growth factors
Product name : rHuman Activin A Active, Human cytokinesand growth factors
Catalog number : RF0010-10
Supplier name : Agren
Data sheet : Ask more or other datasheet now !
Human recombinant protein expressed in Nicotiana benthamiana.
27.4 kDa disulfide-linked homodimers of two βA chains, each containing 116 amino residues
Extinction coefficient :
E0.1% = 1,27 (A 280 nm)
> 97% by SDS-PAGE gel
< 0.04 EU/ µg protein (LAL method)
Activins are homodimers or heterodimers of the various β subunit isoforms, belonging to the TGFβ family. Mature Activin A has two 116 amino acids residues βA subunits (βA-βA). Activin exhibits a wide range of biological activities, including mesoderm induction, neural cell differentiation, bone remodelling, haematopoiesis, and reproductive physiology. Activins plays a key role in the production and regulation of hormones such as FSH, LH, GnRH and ACTH. Cells known to express Activin A include fibroblasts, endothelial cells, hepatocytes, vascular smooth muscle cells, macrophages, keratinocytes, osteoclasts, bone marrow monocytes, prostatic epithelium, neurons, chondrocytes, osteoblasts, Leydig cells, Sertoli cells, and ovarian granulosa cells. As with other members of the super-family, Activins interact with two types of cell surface trans-membrane receptors (Types I and II) which have intrinsic serine/threonine kinase activities in their cytoplasmic domains, Activin type 1 receptors, ACVR1, ACVR1B, ACVR1C and Activin type 2 receptors, ACVR2A, ACVR2B. The biological activity of Activin A can be neutralized by inhibins and by the diffusible TGF-B antagonist, Follistatin.
Lyophilized from a Tris HCl 0.05M buffer at pH 7,4
Produced by transient expression of Activin A in non-transgenic plants. Recombinant human Activin A contains a 6-His-tag at the N-terminal end and is purified by sequential chromatography (FPLC). Contains no animal–derived components or impurities.
Lyophilized protein should be reconstituted in water to a concentration of 50 ng / μl. Due to the protein nature, dimmers and multimers may be observed.
Storage and Stability:
This lyophilized preparation is stable at 2-8º C. For long storage should be kept at -20º C and it is recommended to add a carrier protein (0.1% HSA or BSA). Repeated freezing and thawing is not recommended.
Purity and Serological Identification:
The protein was resolved by SDS polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.
Serological Identification: The protein was electrophoresed under reducing condition on a 15% SDS- polyacrylamide gel, transferred by electroblotting to a NC membrane and visualized by immune-detection with specific antibody Activin A.
Biological Activity: The biological activity of Activin A is measured by its ability to inhibit mouse plasmacytoma cell line (MPC-11) cells proliferation ( [3H]thymidine incorporation).
-Vale W., Hseuh A., Rivier C. and Yu J. (1990). The inhibin/Activin family of hormones and growth factors. In Peptide Growth Factors and their Receptors: Handbook of Experimental Physiology, 95: 211–248. Eds M Sporn & A Roberts. Berlin: Springer-Verlag.
-Schwall R. H., and Lai, C. (1991). Erythroid differentiation bioassys for activin. Methods Enzymol, 198: 340-346.
-Sulyok S., Wankell M., Alzheimer C. and Werner S. (2004). Activin: an important regulator of wound repair, fibrosis, and neuroprotection. Mol. Cell. Endocrinology, 225 (1-2): 127–32.
-Bamberger C., Schärer A., Antsiferova M., Tychsen B., Pankow S., Müller M., Rülicke T., Paus R. and Werner S. (2005). Activin controls skin morphogenesis and wound repair predominantly via stromal cells and in a concentration- dependent manner via keratinocytes. Am. J. Pathol., 167 (3): 733–47.
-Chen Y. G., Wang Q., Lin S. L., Chang C. D., Chuang J., Chung J. and Ying S. Y. (2006). Activin signalling and its role in regulation of cell proliferation, apoptosis, and carcinogenesis. Exp. Biol. Med. (Maywood), 231 (5): 534–44.
-Phillips D. J., Brauman J. N., Mason A. J., Kretser D.M and Hedger M. P.(1999). A sensitive and specific in vitro bioassay for activin using a mouse plasmacytoma cell line, MPC-11. J. of Endocrinology, 162: 111-116.
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