GENTAUR Belgium BVBA BE0473327336 Voortstraat 49, 1910 Kampenhout BELGIUM Tel 0032 16 58 90 45
GENTAUR U.S.A Genprice Inc,Logistics 547 Yurok Circle, SanJose, CA 95123
Tel (408) 780-0908, Fax (408) 780-0908, [email protected]

Index / Agren / rHuman Activin A Active, Human cytokinesand growth factors /Product Detail : RF0010-10 rHuman Activin A Active, Human cytokinesand growth factors
Related keywords:

#RF0010-10 rHuman Activin A Active, Human cytokinesand growth factors

Ask technical file .

  Price : 190   EUR
216   USD
147   GBP
799   Zloty
25478   JPY
1468   NOK
1573   SEK
215   CHF

Product name : rHuman Activin A Active, Human cytokinesand growth factors

Catalog number : RF0010-10

Quantity: 10

Availability: Yes

Supplier name : Agren

ask pdf gentaur products Data sheet : Ask more or other datasheet now !

About this Product :

rHuman Activin A Active, Human cytokinesand growth factors Human samples 80 % of the research is conducted on human samples. Gentaur suppliers human normal cells, cell lines, RNA extracts and lots of antibodies and ELISA kits to Human proteins as well as rHuman Activin A Active, Human cytokinesand growth factors.
http://mybiofast.com/ver.php?search=human+&submit=Search

More Details about

Human recombinant protein expressed in Nicotiana benthamiana. 

Molecular formula:
C600H911N173O174S13

Molecular weight:
27.4  kDa disulfide-linked homodimers of two βA chains, each containing 116 amino residues

p.I:
7,27

Extinction coefficient :
E0.1% = 1,27 (A 280 nm)

Purity assay:
> 97% by SDS-PAGE gel

Endotoxin level:
< 0.04 EU/ µg protein (LAL method)

Animal-free product*

Sequence:
HHHHHHGLECDGKVNICCKKQFFVSFKDIGWNDWIIAPSGYHANYCEGECPSHIAGTSGSSLSFHSTVINHYRMRGHSPFANLKSCCVPTKLRPMSMLYYDDGQNIIKKDIQNMIVEECGCS

Description:
Activins are homodimers or heterodimers of the various β subunit isoforms, belonging to the TGFβ family. Mature Activin A has two  116 amino acids residues  βA  subunits (βA-βA).  Activin  exhibits a  wide range of biological activities, including mesoderm induction, neural cell differentiation, bone remodelling, haematopoiesis, and reproductive physiology. Activins plays a key role in the production and regulation of hormones such as FSH, LH, GnRH and  ACTH.  Cells known to express Activin A include fibroblasts, endothelial cells, hepatocytes, vascular smooth muscle cells, macrophages, keratinocytes, osteoclasts, bone marrow monocytes, prostatic epithelium, neurons, chondrocytes, osteoblasts, Leydig cells, Sertoli cells, and ovarian granulosa cells. As with other members of the super-family, Activins interact with two types of cell surface  trans-membrane receptors  (Types I and II) which have intrinsic serine/threonine kinase  activities in their cytoplasmic domains,  Activin type 1 receptors,  ACVR1,  ACVR1B, ACVR1C    and  Activin type 2 receptors,  ACVR2A, ACVR2B. The biological activity  of Activin A can be neutralized by inhibins and by the diffusible TGF-B antagonist, Follistatin.

Formulation:  
Lyophilized from a Tris HCl 0.05M buffer at pH 7,4

Source: 
Produced by transient expression of Activin A in non-transgenic plants. Recombinant human Activin A contains a 6-His-tag at the N-terminal end and is purified by sequential chromatography (FPLC). Contains no animal–derived components or impurities.

Reconstitution recommendation: 
Lyophilized protein should be reconstituted in water to a concentration of  50  ng /  μl. Due to the protein nature, dimmers and multimers may be observed.

Storage and Stability:
This lyophilized preparation is stable at 2-8º C. For long storage should be kept at  -20º C and it is recommended to add a carrier protein (0.1% HSA or BSA). Repeated freezing and thawing is not recommended.  

Purity and Serological Identification:
The protein was resolved by SDS polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

Serological Identification:
Serological Identification:  The protein was  electrophoresed under reducing condition on a 15% SDS- polyacrylamide gel, transferred by electroblotting to a NC membrane and visualized by  immune-detection with specific antibody Activin A.

Biological Activity: 
Biological Activity:  The biological activity of Activin A  is measured  by its ability to inhibit  mouse plasmacytoma cell line  (MPC-11)  cells proliferation ( [3H]thymidine incorporation).

ED50 ≤5ng/ml

References

-Vale W., Hseuh A., Rivier C.  and Yu J. (1990).  The inhibin/Activin family of hormones and growth factors. In Peptide Growth Factors and their Receptors: Handbook of Experimental Physiology,  95:  211–248. Eds M Sporn & A Roberts. Berlin: Springer-Verlag.

-Schwall  R. H., and  Lai, C. (1991).  Erythroid differentiation bioassys for activin. Methods Enzymol, 198: 340-346.

-Sulyok S., Wankell M., Alzheimer C. and Werner S. (2004). Activin: an important regulator of wound repair,  fibrosis, and neuroprotection. Mol. Cell. Endocrinology, 225 (1-2): 127–32. 

-Bamberger C., Schärer  A.,  Antsiferova M., Tychsen B., Pankow S.,  Müller M., Rülicke T., Paus R. and Werner S. (2005). Activin controls skin morphogenesis and wound repair predominantly via stromal cells and in a concentration- dependent manner via keratinocytes. Am. J. Pathol., 167 (3): 733–47.

 -Chen Y. G., Wang Q., Lin S. L., Chang C. D., Chuang J., Chung J. and  Ying S. Y. (2006). Activin signalling and its role in regulation of cell proliferation, apoptosis, and carcinogenesis. Exp. Biol. Med. (Maywood), 231 (5): 534–44. 

-Phillips D. J., Brauman J. N., Mason A. J., Kretser D.M and Hedger M. P.(1999). A sensitive and specific in vitro bioassay for activin using a mouse plasmacytoma cell line, MPC-11. J. of Endocrinology, 162: 111-116.

Contact us about this product :

Our team will respond you as soon as possible !

Email :
Skype :
Name :
Phone :
address :
Question, Comment :
arrow security gentaurPlease retype this code below :
Agren \ rHuman_Activin_A_Active,_Human_cytokinesand_growth_factors \ RF0010_10
Reload Image

Kits Elisa; taq POLYMERASE

Search in Google:

google

Share this page:
share on twitter rss feedsfacebookgoogle gentaur



Quick order!
Enter catalog number :


Gentaur; yes we can

Related products : rHuman Activin A Active, Human cytokinesand growth factors


Gentaurpub

Pathways :
WP2256: Integrated Pancreatic Cancer Pathway
WP2377: Integrated Pancreatic Cancer Pathway
WP1046: Signaling of Hepatocyte Growth Factor Receptor
WP1065: Endochondral Ossification
WP107: Translation Factors
WP1084: Translation Factors
WP110: Translation Factors
WP1162: Signaling of Hepatocyte Growth Factor Receptor
WP1181: Endochondral Ossification
WP1206: Signaling of Hepatocyte Growth Factor Receptor
WP1220: Translation Factors
WP1235: Signaling of Hepatocyte Growth Factor Receptor
WP1270: Endochondral Ossification
WP1308: Endochondral Ossification
WP1383: Endochondral Ossification
WP149: Translation Factors
WP1493: Carbon assimilation C4 pathway
WP1541: Energy Metabolism
WP1766: Innexins regulate active zone differentiation
WP1789: Binding of RNA by Insulin-like Growth Factor-2 mRNA Binding Proteins (IGF2BPs/IMPs/VICKZs)
WP1815: Factors involved in megakaryocyte development and platelet production
WP1866: NCAM signaling for neurite out-growth
WP1899: Regulation of Insulin-like Growth Factor (IGF) Activity by Insulin-like Growth Factor Binding Proteins (IGFBPs)
WP193: Signaling of Hepatocyte Growth Factor Receptor
WP2107: pathways in human cancer

Related Genes :
[TGFBR1 ALK5 SKR4] TGF-beta receptor type-1 (TGFR-1) (EC 2.7.11.30) (Activin A receptor type II-like protein kinase of 53kD) (Activin receptor-like kinase 5) (ALK-5) (ALK5) (Serine/threonine-protein kinase receptor R4) (SKR4) (TGF-beta type I receptor) (Transforming growth factor-beta receptor type I) (TGF-beta receptor type I) (TbetaR-I)
[ACVR2A ACVR2] Activin receptor type-2A (EC 2.7.11.30) (Activin receptor type IIA) (ACTR-IIA) (ACTRIIA)
[ACVR1B ACVRLK4 ALK4] Activin receptor type-1B (EC 2.7.11.30) (Activin receptor type IB) (ACTR-IB) (Activin receptor-like kinase 4) (ALK-4) (Serine/threonine-protein kinase receptor R2) (SKR2)
[ACVR1C ALK7] Activin receptor type-1C (EC 2.7.11.30) (Activin receptor type IC) (ACTR-IC) (Activin receptor-like kinase 7) (ALK-7)
[ACVR1 ACVRLK2] Activin receptor type-1 (EC 2.7.11.30) (Activin receptor type I) (ACTR-I) (Activin receptor-like kinase 2) (ALK-2) (Serine/threonine-protein kinase receptor R1) (SKR1) (TGF-B superfamily receptor type I) (TSR-I)
[ACVR2B] Activin receptor type-2B (EC 2.7.11.30) (Activin receptor type IIB) (ACTR-IIB)
[BMPR1A ACVRLK3 ALK3] Bone morphogenetic protein receptor type-1A (BMP type-1A receptor) (BMPR-1A) (EC 2.7.11.30) (Activin receptor-like kinase 3) (ALK-3) (Serine/threonine-protein kinase receptor R5) (SKR5) (CD antigen CD292)
[INHBA] Inhibin beta A chain (Activin beta-A chain) (Erythroid differentiation protein) (EDF)
[FOXH1 FAST1 FAST2] Forkhead box protein H1 (Forkhead activin signal transducer 1) (Fast-1) (hFAST-1) (Forkhead activin signal transducer 2) (Fast-2)
[NTRK2 TRKB] BDNF/NT-3 growth factors receptor (EC 2.7.10.1) (GP145-TrkB) (Trk-B) (Neurotrophic tyrosine kinase receptor type 2) (TrkB tyrosine kinase) (Tropomyosin-related kinase B)
[ACVRL1 ACVRLK1 ALK1] Serine/threonine-protein kinase receptor R3 (SKR3) (EC 2.7.11.30) (Activin receptor-like kinase 1) (ALK-1) (TGF-B superfamily receptor type I) (TSR-I)
[PDGFRA PDGFR2 RHEPDGFRA] Platelet-derived growth factor receptor alpha (PDGF-R-alpha) (PDGFR-alpha) (EC 2.7.10.1) (Alpha platelet-derived growth factor receptor) (Alpha-type platelet-derived growth factor receptor) (CD140 antigen-like family member A) (CD140a antigen) (Platelet-derived growth factor alpha receptor) (Platelet-derived growth factor receptor 2) (PDGFR-2) (CD antigen CD140a)
[ACVR2A] Activin receptor type-2A (EC 2.7.11.30) (Activin receptor type IIA) (ACTR-IIA) (ACTRIIA)
[Acvr2a Acvr2] Activin receptor type-2A (EC 2.7.11.30) (Activin receptor type IIA) (ACTR-IIA)
[ACVR2A ACTRII ACVR2] Activin receptor type-2A (EC 2.7.11.30) (Activin receptor type IIA) (ACTR-IIA)
[Acvr2a Actrii Acvr2] Activin receptor type-2A (EC 2.7.11.30) (Activin receptor type IIA) (ACTR-IIA)
[INHBB] Inhibin beta B chain (Activin beta-B chain)
[Acvr1b Acvrlk4 Alk4] Activin receptor type-1B (EC 2.7.11.30) (Activin receptor type IB) (ACTR-IB) (Activin receptor-like kinase 4) (ALK-4) (Serine/threonine-protein kinase receptor R2) (SKR2)
[Acvr1b Alk4] Activin receptor type-1B (EC 2.7.11.30) (Activin receptor type IB) (ACTR-IB) (Activin receptor-like kinase 4) (ALK-4) (Serine/threonine-protein kinase receptor R2) (SKR2)
[Acvr1c Alk7] Activin receptor type-1C (EC 2.7.11.30) (Activin receptor type IC) (ACTR-IC) (Activin receptor-like kinase 7) (ALK-7)
[Acvr1c Alk7] Activin receptor type-1C (EC 2.7.11.30) (Activin receptor type IC) (ACTR-IC) (Activin receptor-like kinase 7) (ALK-7)
[FGF9] Fibroblast growth factor 9 (FGF-9) (Glia-activating factor) (GAF) (Heparin-binding growth factor 9) (HBGF-9)
[FGF1 FGFA] Fibroblast growth factor 1 (FGF-1) (Acidic fibroblast growth factor) (aFGF) (Endothelial cell growth factor) (ECGF) (Heparin-binding growth factor 1) (HBGF-1)
[Acvr2b Actriib] Activin receptor type-2B (EC 2.7.11.30) (Activin receptor type IIB) (ACTR-IIB)
[PDGFD IEGF SCDGFB MSTP036 UNQ1899/PRO4345] Platelet-derived growth factor D (PDGF-D) (Iris-expressed growth factor) (Spinal cord-derived growth factor B) (SCDGF-B) [Cleaved into: Platelet-derived growth factor D, latent form (PDGFD latent form); Platelet-derived growth factor D, receptor-binding form (PDGFD receptor-binding form)]
[IGF2 PP1446] Insulin-like growth factor II (IGF-II) (Somatomedin-A) (T3M-11-derived growth factor) [Cleaved into: Insulin-like growth factor II; Insulin-like growth factor II Ala-25 Del; Preptin]
[PDGFA PDGF1] Platelet-derived growth factor subunit A (PDGF subunit A) (PDGF-1) (Platelet-derived growth factor A chain) (Platelet-derived growth factor alpha polypeptide)
[foxh1 fast-1 fast1 foxh1a] Forkhead box protein H1 (Forkhead activin signal transducer 1) (Fast-1) (xFAST-1) (XFoxH1a)
[FGFR1 BFGFR CEK FGFBR FLG FLT2 HBGFR] Fibroblast growth factor receptor 1 (FGFR-1) (EC 2.7.10.1) (Basic fibroblast growth factor receptor 1) (BFGFR) (bFGF-R-1) (Fms-like tyrosine kinase 2) (FLT-2) (N-sam) (Proto-oncogene c-Fgr) (CD antigen CD331)
[BAMBI NMA] BMP and activin membrane-bound inhibitor homolog (Non-metastatic gene A protein) (Putative transmembrane protein NMA)

Bibliography :