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Index / Arbor / Cortisol Enzyme Immunoassay kit - 1 Strip plate /Product Detail : K003-H1 Cortisol Enzyme Immunoassay kit - 1 Strip plate
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#K003-H1 Cortisol Enzyme Immunoassay kit - 1 Strip plate

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  Price : 267   EUR
303   USD
207   GBP
1123   Zloty
35785   JPY
2062   NOK
2210   SEK
302   CHF

Product name : Cortisol Enzyme Immunoassay kit - 1 Strip plate

Catalog number : K003-H1

Quantity: 1x96 well plate

Availability: Yes

Supplier name : Arbor

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More Details about

FEATURES

  •  
  •  
  • USE - Measure Cortisol in 90 Minutes
  • SAMPLE - Fecal Extracts, Urine, Serum, Plasma, Saliva and TCM
  • SAMPLES/KIT - 40 or 232 in Duplicate
  • CALIBRATED - N-Cal Kit, NIST-Calibrated
  • STABILITY - Stable 4˚C Liquid Reagents

DESCRIPTION

The DetectX® Cortisol Immunoassay kit is designed to quantitatively measure cortisol present in dried fecal extracts, saliva, urine, serum, plasma and culture media samples. This kit measures total cortisol in extracted samples, serum and plasma and free cortisol in saliva and urine. A cortisol standard is provided to generate a standard curve for the assay. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture mouse antibodies. A cortisol-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a monoclonal antibody to cortisol to each well. After an 1 hour incubation the plate is washed and substrate is added. The substrate reacts with the bound cortisol-peroxidase conjugate. After a short incubation the color reaction is read at 450nm.

Cortisol is the primary glucocorticoid produced and secreted by the adrenal cortex. It is often referred to as the “stress hormone” as it is involved in the response to stress and it affects blood pressure, blood sugar levels, and other actions of stress adaptation. Immunologically, cortisol functions as an important anti-inflammatory and plays a role in hypersensitivity, immunosuppression, and disease resistance. In the metabolic aspect, cortisol promotes gluconeogenesis, liver glycogen deposition, and the reduction of glucose utilization. Production of cortisol follows an ACTH-dependent circadian rhythm, with a peak level in the morning and decreasing levels throughout the day. Most serum cortisol, all but about 4%, is bound to proteins including corticosteroid binding globulin and serum albumin. Only free cortisol is available to most receptors and it is through these receptors that physiological processes are modulated. Abnormal cortisol levels are being evaluated for correlation with a variety of different conditions, such as prostate cancer, depression, and schizophrenia. It is already known that abnormal levels of cortisol are involved in Cushing’s Syndrome and Addison’s disease.

Cortisol EIA Standard Curve

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Pathways :
WP142: mRNA processing
WP1493: Carbon assimilation C4 pathway
WP1815: Factors involved in megakaryocyte development and platelet production
WP1819: Formation of Platelet plug
WP1881: Platelet activation triggers
WP1882: Platelet Activation
WP1883: Platelet Adhesion to exposed collagen
WP1884: Platelet Aggregation (Plug Formation)
WP1885: Platelet homeostasis
WP1903: Response to elevated platelet cytosolic Ca2+
WP1970: Glycoprotein VI platelet signaling
WP2340: Thiamine (vitamin B1) biosynthesis and salvage
WP2341: vitamin B1 (thiamin) biosynthesis and salvage pathway
WP2344: vitamin B6 (pyridoxine, pyridoxal, pyridoxamine) biosynthesis and salvage pathway
WP2349: vitamin B3 (niacin), NAD and NADP biosynthesis pathway
WP508: Biosynthesis of Aldosterone and Cortisol
WP626: Abscisic Acid Biosynthesis

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