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5,6-Carboxyfluorescein diacetate (CFDA), Fluorescent Esterase Substrate, 100mg
/Product Detail : M0011 5,6-Carboxyfluorescein diacetate (CFDA), Fluorescent Esterase Substrate, 100mg
Product name : 5,6-Carboxyfluorescein diacetate (CFDA), Fluorescent Esterase Substrate, 100mg
Catalog number : M0011
Supplier name : MarkerGene
Data sheet : Ask more or other datasheet now !
Popular, ultrasensitive, fluorescent substrate for measuring esterase activity in live cells often used in cell viability assays and "Live-Dead" assay systems.
Especially useful for automated cell viability assays because the cleavage product produced upon enzymatic or chemical hydrolysis of the acetate group(s) is carboxyfluorescein. This fluorophore is well-retained inside live cells, while the substrate CFDA is membrane permeant. See the references for additional information about CFDA use in bacterial, plant, yeast and mammalian cell culture. Green emission spectral properties (Abs 488 nm / EM 517 nm) after removal of acetate esters, intracellularly.
Molecular Weight: 460.4
Storage: F, D
[C=Cold D=Desiccated F=Frozen L=Light Sensitive RT=Room Temperature]
Absorption: (in nm) 300
Extinction (103): 63
Molecular Formula: C25H16O9
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