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Index / MarkerGene / MarkerGeneTM beta-Glucuronidase (GUS) Reporter Gene Activity Detection Kit, Allows for quantitative measurement of GUS enzyme activity in transformed plants though the use of a fluorogenic substrate, /Product Detail : M0877 MarkerGeneTM beta-Glucuronidase (GUS) Reporter Gene Activity Detection Kit, Allows for quantitative measurement of GUS enzyme activity in transformed plants though the use of a fluorogenic substrate,
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MarkerGeneTM beta-Glucuronidase (GUS) Reporter Gene Activity Detection Kit, Allows for quantitative measurement of GUS enzyme activity in transformed plants though the use of a fluorogenic substrate,

 Price: 423   EUR
480   USD
328   GBP

Product name : MarkerGeneTM beta-Glucuronidase (GUS) Reporter Gene Activity Detection Kit, Allows for quantitative measurement of GUS enzyme activity in transformed plants though the use of a fluorogenic substrate,

Catalog number : M0877

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Supplier name : MarkerGene

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MarkerGeneTM beta-Glucuronidase (GUS) Reporter Gene Activity Detection Kit, Allows for quantitative measurement of GUS enzyme activity in transformed plants though the use of a fluorogenic substrate, antibody storage Gentaur recommends for long therm storage to freeze at -24 C. For short time storage up to 30 days we suggest fridge storage at 1 to 10 C. Prevent multiple freeze taw cycles of MarkerGeneTM beta-Glucuronidase (GUS) Reporter Gene Activity Detection Kit, Allows for quantitative measurement of GUS enzyme activity in transformed plants though the use of a fluorogenic substrate, .
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Description:
Allows for quantitative measurement of GUS enzyme activity in transformed plants through use of a fluorogenic substrate.

Application:
The β-glucuronidase (GUS) enzyme from E. coli (EC 3.2.1.31) has been well documented to provide desirable characteristics as a marker gene in transformed plants. The GUS reporter gene system has many advantages including stable expression of E. coli GUS enzyme, no interference with normal plant metabolism, and low intrinsic GUS activity in higher plants. The enzyme is also capable of tolerating amino-terminal additions, making it useful for study of plant organelle transport [1,2,3,4]. Various β-glucuronic acid substrates are available for detection of GUS expression, all of which contain the sugar D-glucopyranosiduronic acid attached by glycosidic linkage to a hydroxyl group of a chromogenic, fluorogenic, or other detectable molecule [5]. The most widely used fluorogenic substrate for detection of β-glucuronidase activity in vitro is 4-methylumbelliferyl β-D-glucuronide (4-MUG). Upon hydrolysis by GUS, the fluorochrome 4-methylumbelliferone (7-hydroxy-4-methyl coumarin) is produced along with sugar glucuronic acid [5]. It is used in this fluorescent activity detection test. Please contact our technical services department for further information.

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