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Index / Trevigen / Stem Cell Mesenchymal Stem Cell Adipogenic Differentiation Kit /Product Detail : 5010-024-K Stem Cell Mesenchymal Stem Cell Adipogenic Differentiation Kit
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#5010-024-K Stem Cell Mesenchymal Stem Cell Adipogenic Differentiation Kit

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  Price : 393   EUR
446   USD
305   GBP
1653   Zloty
52675   JPY
3036   NOK
3253   SEK
445   CHF

Product name : Stem Cell Mesenchymal Stem Cell Adipogenic Differentiation Kit

Catalog number : 5010-024-K

Quantity: 24 wells

Availability: Yes

Supplier name : Trevigen

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Mesenchymal Stem Cell Adipogenic Differentaition Kit
Catalog #: 5010-024-K

Cultrex® Mesenchymal Stem Cell Adipogenic Differentiation Kit

Adipogenesis is the process by which fat is formed.(1) Fat formation plays a role in obesity, cardiovascular disease, and metabolic disorders.(1) It also has a role in the maintenance of the bone marrow cavity. Adipocytes are derived from mesenchymal stem cells (MSC), which are a self-renewing population of multipotent cells present in bone marrow and many other adult tissues that can differentiate into multiple lineage-specific cells that form bone, fat, cartilage, muscle and tendon.(2-5) Differentiation of primary cells, specifically MSC, into adipocytes can be a useful tool for understanding the mechanisms involved in adipogenesis leading eventually to interventions designed to prevent obesity.

Trevigen’s adipogenic differentiation kit follows traditional methods of adipogenic differentiation,(6) by growing MSC in medium supplemented with insulin, isobutyl methyl xanthine (IBMX), indomethacin and dexamethasone. The kit will induce adipogenic differentiation of MSC within 14-17 days. Adipogenic differentiation is detected by staining with Oil Red O. Oil Red O will stain lipid containing vacuoles in mesenchymal stem cells that have undergone adipogenic differentiation.

FEATURES:

Optimized reagents for the differentiation of Mesenchymal Stem Cells into Adipocytes. Sufficient reagents to differentiate one 24 well plate and stain two 24 well plates. The amount of differentiation can be quantified.

APPLICATIONS :

Tool for understanding mechanisms involved in adipogenesis eventually leading to interventions designed to prevent obesity.

REFERENCES:

1. Kiess W, Petzold S, Topfer M, Garten A, Bluher, Kapellen T, Korner A, Kratzsch J 2008 Adipocytes and Adipose Tissue. Best Practice and Research Clinical Endocrinology and Metabolism 22(1):135-153.
2. Phinney DG AND Prockop DJ 2007. Concise Review: Mesenchymal Stem/Multipotent Stromal Cells: The State of Transdifferentiation and Models of Tissue Repair-Current Views. Stem Cells 25:2896-2902
3. Kolf CM, Cho E, Tuan RS. 2007. Biology of Adult Mesenchymal Stem Cells: Regulation of Niche, Self-Renewal and Differentiation. Arthritis Research and Therapy 9(1): 204-214
4. Javazon EH, Colter DC, Schwarz EJ, Prockop DJ. 2001. Rat Marrow Stromal Cells are More Sensative to Plating Density and Expand More Rapidly from Single-Cell-Derived Colonies than Human Marrow Stromal Cells. Stem Cells 19:219-225
5. Li Yi, McIntosh K, Chen J, Zhang C, Gao, Q, Borneman J, Ragniski K, Mitchell J, Shen L, Zhang J, Lu D, Chopp M. Allogeneic bone marrow stromal cells promote glial-axonal remodeling without immunologic sensitization after stroke in rats. Experimental Neurology 198(2):313-25.
6. Reger RL, Tucker AH, Wolfe MR. 2008. Differentiation and Characterization of Human MSCs. In Prockop DJ, Phinney DG, Bunnell BA (eds) Methods in Molecular Biology Mesenchymal Stem Cells: Methods and Protocols vol 449. Humana Press, Totawa, NJ, USA pp 93-107

 

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Pathways :
WP2012: miRs in Muscle Cell Differentiation
WP2023: Cell Differentiation - meta
WP2029: Cell Differentiation - Index
WP2076: miRs in Muscle Cell Differentiation
WP2082: Cell Differentiation - Index
WP2276: Glial Cell Differentiation
WP1011: T Cell Receptor Signaling Pathway
WP1025: B Cell Receptor Signaling Pathway
WP1043: Calcium Regulation in the Cardiac Cell
WP1069: Integrin-mediated cell adhesion
WP1078: G1 to S cell cycle control
WP1083: Cell cycle
WP1130: T Cell Receptor Signaling Pathway
WP1144: B Cell Receptor Signaling Pathway
WP1159: Calcium Regulation in the Cardiac Cell
WP1185: Integrin-mediated cell adhesion
WP1195: G1 to S cell cycle control
WP1200: Cell cycle
WP1345: T Cell Receptor Signaling Pathway
WP1354: B Cell Receptor Signaling Pathway
WP1365: Calcium Regulation in the Cardiac Cell
WP1386: Integrin-mediated cell adhesion
WP1393: Cell cycle
WP1411: Cell Division: First embryonic mitosis
WP1775: Cell Cycle Checkpoints

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[KIT SCFR] Mast/stem cell growth factor receptor Kit (SCFR) (EC 2.7.10.1) (Piebald trait protein) (PBT) (Proto-oncogene c-Kit) (Tyrosine-protein kinase Kit) (p145 c-kit) (v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog) (CD antigen CD117)
[KITLG MGF SCF] Kit ligand (Mast cell growth factor) (MGF) (Stem cell factor) (SCF) (c-Kit ligand) [Cleaved into: Soluble KIT ligand (sKITLG)]
[Kit Sl] Mast/stem cell growth factor receptor Kit (SCFR) (EC 2.7.10.1) (Proto-oncogene c-Kit) (Tyrosine-protein kinase Kit) (CD antigen CD117)
[Kitlg Kitl Mgf Sl Slf] Kit ligand (Hematopoietic growth factor KL) (Mast cell growth factor) (MGF) (Steel factor) (Stem cell factor) (SCF) (c-Kit ligand) [Cleaved into: Soluble KIT ligand (sKITLG)]
[Kitlg Kitl Mgf] Kit ligand (Hematopoietic growth factor KL) (Mast cell growth factor) (MGF) (Stem cell factor) (SCF) (c-Kit ligand) [Cleaved into: Soluble KIT ligand (sKITLG)]
[KITLG MGF] Kit ligand (Mast cell growth factor) (MGF) (Stem cell factor) (SCF) (c-Kit ligand) [Cleaved into: Soluble KIT ligand (sKITLG); Processed kit ligand]
[KIT] Mast/stem cell growth factor receptor Kit (SCFR) (EC 2.7.10.1) (Proto-oncogene c-Kit) (Tyrosine-protein kinase Kit) (CD antigen CD117)
[KIT] Mast/stem cell growth factor receptor Kit (SCFR) (EC 2.7.10.1) (Proto-oncogene c-Kit) (Tyrosine-protein kinase Kit) (CD antigen CD117)
[KIT] Mast/stem cell growth factor receptor Kit (SCFR) (EC 2.7.10.1) (Proto-oncogene c-Kit) (Tyrosine-protein kinase Kit) (CD antigen CD117)
[KIT] Mast/stem cell growth factor receptor Kit (SCFR) (EC 2.7.10.1) (Proto-oncogene c-Kit) (Tyrosine-protein kinase Kit)
[KITLG SCF] Kit ligand (Mast cell growth factor) (MGF) (Stem cell factor) (SCF) (c-Kit ligand) [Cleaved into: Processed kit ligand]
[KITLG SCF] Kit ligand (Mast cell growth factor) (MGF) (Stem cell factor) (SCF) (c-Kit ligand) [Cleaved into: Soluble KIT ligand (sKITLG)]
[KIT] Mast/stem cell growth factor receptor Kit (SCFR) (EC 2.7.10.1) (Proto-oncogene c-Kit) (Tyrosine-protein kinase Kit) (CD antigen CD117)
[KIT] Mast/stem cell growth factor receptor Kit (SCFR) (EC 2.7.10.1) (Proto-oncogene c-Kit) (Tyrosine-protein kinase Kit) (CD antigen CD117)
[KIT] Mast/stem cell growth factor receptor Kit (SCFR) (EC 2.7.10.1) (Proto-oncogene c-Kit) (Tyrosine-protein kinase Kit) (CD antigen CD117)
[kita kit sparse] Mast/stem cell growth factor receptor kita (SCFR) (EC 2.7.10.1) (Tyrosine-protein kinase Kit)
[Kit KIT rCG_56893] Mast/stem cell growth factor receptor (EC 2.7.10.1)
[NGRN FI58G HT020] Neugrin (Mesenchymal stem cell protein DSC92) (Neurite outgrowth-associated protein) (Spinal cord-derived protein FI58G)
[kit krk1] Mast/stem cell growth factor receptor-related protein Kit (EC 2.7.10.1) (Kit-related kinase 1) (xKrk1) (Tyrosine-protein kinase kit)
[KIT] Mast/stem cell growth factor receptor (EC 2.7.10.1)
[KIT] Mast/stem cell growth factor receptor (EC 2.7.10.1)
[KIT CK820_G0003210] Mast/stem cell growth factor receptor (EC 2.7.10.1)
[KIT hCG_22160] Mast/stem cell growth factor receptor (EC 2.7.10.1)
[Kit] Mast/stem cell growth factor receptor (EC 2.7.10.1)
[KIT] Mast/stem cell growth factor receptor (EC 2.7.10.1)
[KIT] Mast/stem cell growth factor receptor (EC 2.7.10.1)
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Bibliography :
[30600670] [Effects of adipose-derived stem cell released exosomes on proliferation, migration, and tube-like differentiation of human umbilical vein endothelial cells].
[30558911] Differentiation potential of the cells in the macula flava of the human vocal fold mucosa.
[30460778] Inhibiting transforming growth factor-β signaling regulates in vitro maintenance and differentiation of bovine bone marrow mesenchymal stem cells.
[30230583] The regulatory roles of VEGF-Notch signaling pathway on aplastic anemia with kidney deficiency and blood stasis.
[30210548] Comparative Analysis of Human Adipose-Derived Mesenchymal Stem Cells from Orbital and Abdominal Fat.
[30139016] [Effects and mechanism of apolipoprotein A5 on adipogenic differentiation of human adipose-derived mesenchymal stem cells].
[30058710] Ipriflavone promotes osteogenesis of MSCs derived from osteoporotic rats.
[29798615] [Comparison of biological characteristics between bone marrow mesenchymal stem cells and anterior cruciate ligament derived mesenchymal stem cells in rats].
[29649411] The In Vitro Differentiation of GDNF Gene-Engineered Amniotic Fluid-Derived Stem Cells into Renal Tubular Epithelial-Like Cells.
[29649060] Liposuction Aspirate Fluid Adipose-Derived Stem Cell Injection and Secondary Healing in Fingertip Injury: A Pilot Study.