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5_HT (Serotonin) _ BSA Conjugate Control Control Peptide
/Product Detail : 20081 5_HT (Serotonin) _ BSA Conjugate Control Control Peptide
Product name : 5_HT (Serotonin) _ BSA Conjugate Control Control Peptide
Catalog number : 20081
Quantity: 0.05 mg
Supplier name : ACR
Data sheet : Ask more or other datasheet now !
DESCRIPTION: Highly purifi ed type II collagen (treated with 3M guanidine, DEAE-cellulose and Na2HPO4)
APPLICATION: Can be used for various purposes, such as a standard for collagen analysis by SDS-gel, substrate for MMP-1, MMP-8, and MMP-13, and as an antigen for immunizing animals for developing anti-sheep type II collagen
QUANTITY: 5 mg
FORM: Lyophilized powder
SOURCE: Sheep articular cartilage
MOLECULAR WEIGHT: 300 Kd
PURITY: >95% by SDS-PAGE, free of pepsin and proteoglycans (Figure 1)
SOLUBILITY: Soluble in acidic buffer (maximum 4 mg/ml), but diffi cult to dissolve in neutral buffer. In order to dissolve this product in a neutral buffer, use 0.01M or 0.05M acetic acid to dissolve the collagen at 1-4 mg/ml and then add this collagen solution to a desired neutral buffer such as 2-10X Tris-NaCl (the fi nal concentration of NaCl: 0.15-0.2M) or dialyze against 1X neutral buffer at 4°C.
Note 1: To avoid fi bril formation under neutral conditions, keep solution on ice.
Note 2: The physicochemical property of type II collagen differs from type I collagen, and type II collagen
dissolved in a neutral buffer does not form stable gel with incubation at 37°C. In order to prepare
a type II collagen gel, additional components such as type I collagen or other substances might
STORAGE TEMPERATURE: 4°C in the dark
STABILITY: 1 year
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