GENTAUR Belgium BVBA BE0473327336 Voortstraat 49, 1910 Kampenhout BELGIUM Tel 0032 16 58 90 45
GENTAUR U.S.A Genprice Inc,Logistics 547 Yurok Circle, SanJose, CA 95123
Tel (408) 780-0908, Fax (408) 780-0908, [email protected]

Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, Gentaur another in time delivery

Pubmed ID: 18669648
Publication Date: 2008/07/31

A quantitative atlas of mitotic phosphorylation.


The eukaryotic cell division cycle is characterized by a sequence of orderly and highly regulated events resulting in the duplication and separation of all cellular material into two newly formed daughter cells. Protein phosphorylation by cyclin-dependent kinases (CDKs) drives this cycle. To gain further insight into how phosphorylation regulates the cell cycle, we sought to identify proteins whose phosphorylation is cell cycle regulated. Using stable isotope labeling along with a two-step strategy for phosphopeptide enrichment and high mass accuracy mass spectrometry, we examined protein phosphorylation in a human cell line arrested in the G(1) and mitotic phases of the cell cycle. We report the identification of >14,000 different phosphorylation events, more than half of which, to our knowledge, have not been described in the literature, along with relative quantitative data for the majority of these sites. We observed >1,000 proteins with increased phosphorylation in mitosis including many known cell cycle regulators. The majority of sites on regulated phosphopeptides lie in [S/T]P motifs, the minimum required sequence for CDKs, suggesting that many of the proteins may be CDK substrates. Analysis of non-proline site-containing phosphopeptides identified two unique motifs that suggest there are at least two undiscovered mitotic kinases.
Authors: Dephoure Noah , Zhou Chunshui , Villén Judit , Beausoleil Sean A , Bakalarski Corey E , Elledge Stephen J , Gygi Steven P ,

Reference:

  1. ncbi.nlm.nih.gov. [Last access 2008/07/31].

Related products :

Catalog number Product name Quantity
EERK-100 EnzyFluo™ ERK Phosphorylation Assay Kit, Quantitative fluorescent immunoenzymatic assay for ERK1_2 phosphorylation status in cultured cells. This simple assay eliminates the need for cell lysate prepa 100tests
26-354 PBK is a serine_threonine kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activ 0.05 mg
30-460 PBK is a serine_threonine kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activ 0.05 mg
EIAAB26169 C13orf37,Homo sapiens,Human,Mitotic-spindle organizing protein 1,Mitotic-spindle organizing protein associated with a ring of gamma-tubulin 1,MOZART1,MZT1
EIAAB26173 FAM128A,Homo sapiens,Human,Mitotic-spindle organizing protein 2A,Mitotic-spindle organizing protein associated with a ring of gamma-tubulin 2A,MOZART2A,MZT2A