GENTAUR Belgium BVBA BE0473327336 Voortstraat 49, 1910 Kampenhout BELGIUM Tel 0032 16 58 90 45
GENTAUR U.S.A Genprice Inc,Logistics 547 Yurok Circle, SanJose, CA 95123
Tel (408) 780-0908, Fax (408) 780-0908, [email protected]

Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, Gentaur another in time delivery

Pubmed ID: 19144319
Publication Date: //

The phagosomal proteome in interferon-gamma-activated macrophages.


The ability of macrophages to clear pathogens and elicit a sustained immune response is regulated by various cytokines, including interferon-gamma (IFN-gamma). To investigate the molecular mechanisms by which IFN-gamma modulates phagosome functions, we profiled the changes in composition, abundance, and phosphorylation of phagosome proteins in resting and activated macrophages by using quantitative proteomics and bioinformatics approaches. We identified 2415 phagosome proteins together with 2975 unique phosphorylation sites with a high level of sensitivity. Using network analyses, we determined that IFN-gamma delays phagosomal acquisition of lysosomal hydrolases and peptidases for the gain of antigen presentation. Furthermore, this gain in antigen presentation is dependent on phagosomal networks of the actin cytoskeleton and vesicle-trafficking proteins, as well as Src kinases and calpain proteases. Major histocompatibility complex class I antigen-presentation assays validated the molecular participation of these networks in the enhanced capacity of IFN-gamma-activated macrophages to crosspresent exogenous antigens to CD8(+) T cells.
Authors: Trost Matthias , English Luc , Lemieux Sébastien , Courcelles Mathieu , Desjardins Michel , Thibault Pierre ,

Reference:

  1. ncbi.nlm.nih.gov. [Last access //].

Related products :

Catalog number Product name Quantity
20-663-48022 Interferon-gamma (mAHuIFNg) - Mouse Anti Human Interferon-gamma (mAHuIFNg); IFN-gamma; Immune interferon Monoclonal 0.5 mg
20-663-48022 Interferon-gamma (mAHuIFNg) - Mouse Anti Human Interferon-gamma (mAHuIFNg); IFN-gamma; Immune interferon Monoclonal 1 mg
orb82799 Mouse IFN gamma protein Interferon gamma (IFNG) is a cytokine produced primarily by T-lymphocytes and Natural Killer cells activated by specific antigens or mitogens. Mature IFNG exists as noncovalent 1 mg
18-783-78408 RABBIT ANTI HUMAN INTERFERON GAMMA Biotin - INTERFERON GAMMA; IFN-gamma Polyclonal 0.05 mg
18-783-78407 RABBIT ANTI HUMAN INTERFERON GAMMA - INTERFERON GAMMA; IFN-gamma Polyclonal 0.1 mg