Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, Gentaur another in time delivery

Pubmed ID :27565731
Publication Date : //

mTOR inhibition sensitizes ONC201-induced anti-colorectal cancer cell activity.


We here tested the anti-colorectal cancer (CRC) activity by a first-in-class small molecule TRAIL inducer ONC201. The potential effect of mTOR on ONC201's actions was also examined. ONC201 induced moderate cytotoxicity against CRC cell lines (HT-29, HCT-116 and DLD-1) and primary human CRC cells. Significantly, AZD-8055, a mTOR kinase inhibitor, sensitized ONC201-induced cytotoxicity in CRC cells. Meanwhile, ONC201-induced TRAIL/death receptor-5 (DR-5) expression, caspase-8 activation and CRC cell apoptosis were also potentiated with AZD-8055 co-treatment. Reversely, TRAIL sequestering antibody RIK-2 or the caspase-8 specific inhibitor z-IETD-fmk attenuated AZD-8055 plus ONC201-induced CRC cell death. Further, mTOR kinase-dead mutation (Asp-2338-Ala) or shRNA knockdown significantly sensitized ONC201's activity in CRC cells, leading to profound cell death and apoptosis. On the other hand, expression of a constitutively-active S6K1 (T389E) attenuated ONC201-induced CRC cell apoptosis. For the mechanism study, we showed that ONC201 blocked Akt, but only slightly inhibited mTOR in CRC cells. Co-treatment with AZD-8055 also concurrently blocked mTOR activation. These results suggest that mTOR could be a primary resistance factor of ONC201 in CRC cells.

Authors : Jin Zhe-Zhu , Wang Wei , Fang Di-Long , Jin Yong-Jun ,

Related products :

Catalog number Product name Quantity
orb61373 Temsirolimus Temsirolimus, a rapamycin (sirolimus) derivative, also known as CCI-779, is an mTOR inhibitor with anti-cancer activity. For research use only. 50 mg
9-80029 Human Cancer PrimaCell™6: Colorectal Tumor Cells, primary cell culture 1 x 100 ml
9-47029 Human Cancer PrimaCell™6: Colorectal Tumor Cells, primary cell culture set
4-28029 Human Cancer PrimaCell™6: Colorectal Tumor Cells, primary cell culture Kit
9-46029 Human Cancer PrimaCell™6: Colorectal Tumor Cells, primary cell culture 5 x 100 ml
2-96029 Human Cancer PrimaCell™6: Colorectal Tumor Cells, primary cell culture Kit
30-121 C21orf33 inhibits both estrogen- and heregulin-beta1-stimulated growth of breast cancer cells, and further suggests that growth inhibition induced in these cells by all-trans retinoic acid occurs via 0.1 mg
ICTS-SER Cancer samples: Colorectal Cancer Serum-Adenocarcinoma Each
Tissues-123-COLORECTAL CANCER Human Tissues - COLORECTAL CANCER 1unit
30-397 RSU1 is a protein that is involved in the Ras signal transduction pathway, growth inhibition, and nerve-growth factor induced differentiation processes, as determined in mouse and human cell line stud 0.1 mg
P-4003-96 EpiQuik HAT Activity per Inhibition Assay Kit 96 assays
P-4003-48 EpiQuik HAT Activity per Inhibition Assay Kit 48 assays
BC000110 Colorectal cancer tissue array, 85 cases_170 cores; ihc Anti-Actin confirmed
orb61265 MK-4827 MK-4827 displays excellent PARP 1 and 2 inhibition with IC50 = 3.8 and 2.1 nM, resp., and in a whole cell assay, it inhibited PARP activity with EC50 = 4 nM and inhibited proliferation of canc 5 mg
P-3019-48 EpiQuik DNA Demethylase Activity per Inhibition Assay Kit 48 assays
P-3087-48 Epigenase 5mC-Hydroxylase TET Activity per Inhibition Assay Kit Fluorometric 48 assays
P-4002-48 EpiQuik HDAC Activity per Inhibition Assay Kit Colorimetric 48 assays
P-3008-48 EpiQuik DNA Demethylase Activity per Inhibition Assay Ultra Kit 48 assays
P-4001-96 EpiQuik HDAC Activity per Inhibition Assay Kit Fluorometric 96 assays
P-4002-96 EpiQuik HDAC Activity per Inhibition Assay Kit Colorimetric 96 assays
P-3086-48 Epigenase 5mC-Hydroxylase TET Activity per Inhibition Assay Kit Colorimetric 48 assays
P-3021-48 EpiQuik MBD2 Binding Activity per Inhibition Assay Kit 48 assays
P-4001-48 EpiQuik HDAC Activity per Inhibition Assay Kit Fluorometric 48 assays
P-3001-1 EpiQuik DNA Methyltransferase DNMT Activity per Inhibition Assay Kit 48 assays
ICTS-SER-1ml Colorectal Cancer SerumAdenocarcinoma 1ml 1ml


https://antibody-antibodies.com/ | https://gentaur.com/ | https://gen-script.com/ | https://diagenox.com/ | https://clonagen.com/ | http://gentaursearch.com/ | http://gentaurpub.com/ | https://gentaur-online.com/ | http://anti-anti-pdf.com/ | http://gentaur-worldwide.com/

 

Gentaur Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
[email protected] | Gentaur | Gentaur





Gentaur Ltd.
Unicorn House, Station Cl
Hertfordshire, Potters Bar EN6 1TL
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
[email protected] | Gentaur | Gentaur

 

 




Gentaur France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017
RIB 30004 00187 00010092253 10
BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG
IBAN FR76 3000 4001 8700 0100 9225 310
[email protected] | Gentaur | Gentaur

Gentaur GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: +49 0241 40 08 90 86, +49 0241 95 78 94 78, +49 0241 40 08 90 86
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
[email protected] | Gentaur | Gentaur

Gentaur U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
[email protected]

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




Gentaur Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
[email protected] | Gentaur | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






Gentaur Spain
tel:0911876558
[email protected] | Gentaur | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: [email protected] | Gentaur | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

Gentaur Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

[email protected] | Gentaur | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

Gentaur Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
[email protected] | Gentaur | Gentaur