GENTAUR Belgium BVBA BE0473327336 Voortstraat 49, 1910 Kampenhout BELGIUM Tel 0032 16 58 90 45
GENTAUR U.S.A Genprice Inc,Logistics 547 Yurok Circle, SanJose, CA 95123
Tel (408) 780-0908, Fax (408) 780-0908, [email protected]

Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, Gentaur another in time delivery

Pubmed ID: 28087261
Publication Date: 2016/12/25

DNA aptamer probes for detection of estrogen receptor α positive carcinomas.


Estrogen receptor alpha (ERα) also known as NR3A1 (nuclear receptor subfamily 3, group A, member 1) is a ligand-activated transcription factor. It is an important biomarker for breast cancer metastasis. In the present study, we report a novel DNA aptamer candidate against estrogen receptor (ER) alpha structure. The enriched aptamer candidate was obtained after 14 iterative cycles of in vitro protein-SELEX process. Isothermal calorimetry study suggests the nanomolar sensitivity of the candidate ER_Apt1 to its target protein. Fluorescence- and chemiluminescence-binding assays confirm the specificity of the candidate aptamer to ER alpha positive breast cancer cell line. Comparative analysis of ER_Apt1 to ER alpha monoclonal antibody was also performed to analyze the expression of ER alpha in various malignant cancer cell line. Cytochemical and immunohistochemistry assay indicates its potential use as a diagnostic agent against ERα positive carcinomas. The nucleotide aptamer sequences described in the present study can be used for the detection, treatment, prophylaxis and diagnosis of ERα-related disorder.
Authors: Sett Arghya , Borthakur Bibhuti Bhusan , Sharma Jagannath Dev , Kataki Amal Chandra , Bora Utpal ,

Reference:

  1. ncbi.nlm.nih.gov. [Last access 2016/12/25].

Related products :

Catalog number Product name Quantity
PC-ESTRA Estrogen Receptor alpha Positive Control 5 Applications
PC-ESTRB Rabbit Estrogen Receptor beta Antibody Positive Control 5 Applications
PC-ESTRA Rabbit Estrogen Receptor alpha Antibody Positive Control 5 Applications
52-0025 CISH Poly HRP Detection Kit for DIG Labeled Probes 20 tests
K060111 In Situ Hybridization Detection System (AP), for Biotinylated Probes 150 tests