Pubmed ID: 30401749
Publication Date: 2018/11/06

Activation of hepatocyte growth factor/MET signaling initiates oncogenic transformation and enhances tumor aggressiveness in the murine prostate.

Emerging evidence has shown that the hepatocyte growth factor (HGF) and its receptor, MET proto-oncogene, receptor tyrosine kinase (MET), promote cell proliferation, motility, morphogenesis, and angiogenesis. Whereas up-regulation of MET expression has been observed in aggressive and metastatic prostate cancer, a clear understanding of MET function in prostate tumorigenesis remains elusive. Here, we developed a conditional transgenic mouse strain, , to mimic human prostate cancer cells with increased MET expression in the prostatic luminal epithelium. We found that these mice develop prostatic intraepithelial neoplasia after HGF administration. To further assess the biological role of MET in prostate cancer progression, we bred compound mice, in which transgenic expression and deletion of the tumor suppressor gene occurred simultaneously only in prostatic epithelial cells. These compound mice exhibited accelerated prostate tumor formation and invasion as well as increased metastasis compared with mice. Moreover, prostatic sarcomatoid carcinomas and lesions resembling the epithelial-to-mesenchymal transition developed in tumor lesions of the compound mice. RNA-Seq and qRT-PCR analyses revealed a robust enrichment of known tumor progression and metastasis-promoting genes in samples isolated from compound mice compared with those from littermate controls. HGF-induced cell proliferation and migration also increased in mouse embryonic fibroblasts (MEFs) from animals with both transgene expression and deletion compared with null MEFs. The results from these newly developed mouse models indicate a role for MET in hastening tumorigenesis and metastasis when combined with the loss of tumor suppressors.

Authors: Mi Jiaqi , Hooker Erika , Balog Steven , Zeng Hong , Johnson Daniel T , He Yongfeng , Yu Eun-Jeong , Wu Huiqing , Le Vien , Lee Dong-Hoon , Aldahl Joseph , Gonzalgo Mark L , Sun Zijie ,

Reference:

Related products :

Catalog number	Product name	Quantity
25-948	Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growt	0.05 mg
30-667	LIN9 belongs to the lin-9 family and acts as a tumor suppressor. It inhibits DNA synthesis. Its ability to inhibit oncogenic transformation is mediated through its association with RB1. LIN9 plays a r	0.05 mg
30-666	LIN9 belongs to the lin-9 family and acts as a tumor suppressor. It inhibits DNA synthesis. Its ability to inhibit oncogenic transformation is mediated through its association with RB1. LIN9 plays a r	0.05 mg
31-002	CFLAR might contribute to the carcinogenesis and aggressiveness of endometrial carcinoma and might be a useful prognostic factor in the tumor. CFLAR is specially overexpressed in colon cancers and it	0.1 mg
30-268	NPDC1 suppresses oncogenic transformation in neural and non-neural cells and down-regulates neural cell proliferation. It might be involved in transcriptional regulation.	0.1 mg