GENTAUR Belgium BVBA BE0473327336 Voortstraat 49, 1910 Kampenhout BELGIUM Tel 0032 16 58 90 45
GENTAUR U.S.A Genprice Inc,Logistics 547 Yurok Circle, SanJose, CA 95123
Tel (408) 780-0908, Fax (408) 780-0908, [email protected]

Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, Gentaur another in time delivery

Pubmed ID: 31347661
Publication Date: //

Influence of metabolic status and genetic merit for fertility on proteomic composition of bovine oviduct fluid†.


The oviduct plays a crucial role in fertilization and early embryo development providing the microenvironment for oocyte, spermatozoa, and early embryo. Since dairy cow fertility declined steadily over the last decades, reasons for early embryonic loss have gained increasing interest. Analyzing two animal models, this study aimed to investigate the impact of genetic predisposition for fertility and of metabolic stress on the protein composition of oviduct fluid. A metabolic model comprised maiden Holstein heifers and postpartum lactating (Lact) and non-lactating (Dry) cows, while a genetic model consisted of heifers from the Montbéliarde breed and Holstein heifers with low- and high-fertility index. In a holistic proteomic analysis of oviduct fluid from all groups using nano-liquid chromatography tandem-mass spectrometry analysis and label-free quantification, we were able to identify 1976 proteins, among which 143 showed abundance alterations in the pairwise comparisons within both models. Most differentially abundant proteins were revealed between low fertility Holstein and Montbéliarde (52) in the genetic model and between lactating and maiden Holstein (19) in the metabolic model, demonstrating a substantial effect of genetic predisposition for fertility and metabolic stress on the oviduct fluid proteome. Functional classification of affected proteins revealed actin binding, translation, and immune system processes as prominent gene ontology (GO) clusters. Notably, Actin-related protein 2/3 complex subunit 1B and the three immune system-related proteins SERPIND1 protein, immunoglobulin kappa locus protein, and Alpha-1-acid glycoprotein were affected in both models, suggesting that abundance changes of immune-related proteins in oviduct fluid play an important role for early embryonic loss.
Authors: Gegenfurtner Katrin , Fröhlich Thomas , Kösters Miwako , Mermillod Pascal , Locatelli Yann , Fritz Sébastien , Salvetti P , Forde Niamh , Lonergan Patrick , Wolf Eckhard , Arnold Georg J ,

Reference:

  1. ncbi.nlm.nih.gov. [Last access //].

Related products :

Catalog number Product name Quantity
IVL05-100ML Cell Culture Media,Bovine IVF Media,SOF,Synthetic Oviduct Fluid. 100ml
EIAAB29370 Bos taurus,Bovine,Estrogen-dependent oviduct protein,OGP,OVGP1,Oviductal glycoprotein,Oviductin,Oviduct-specific glycoprotein
EIAAB29373 Estrogen-dependent oviduct protein,OGP,OVGP1,Oviductal glycoprotein,Oviductin,Oviduct-specific glycoprotein,Pig,POSP-E3,Sus scrofa
EIAAB29371 Chit5,Estrogen-dependent oviduct protein,Mouse,Mus musculus,Ogp,Ovgp1,Oviductal glycoprotein,Oviductin,Oviduct-specific glycoprotein
EIAAB29372 Estrogen-dependent oviduct protein,Homo sapiens,Human,MUC9,Mucin-9,OGP,OVGP1,Oviductal glycoprotein,Oviductin,Oviduct-specific glycoprotein