GENTAUR Belgium BVBA BE0473327336 Voortstraat 49, 1910 Kampenhout BELGIUM Tel 0032 16 58 90 45
GENTAUR U.S.A Genprice Inc,Logistics 547 Yurok Circle, SanJose, CA 95123
Tel (408) 780-0908, Fax (408) 780-0908, [email protected]

Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, Gentaur another in time delivery

Pubmed ID: 31505074
Publication Date: //

Increased expression of GAB1 promotes inflammation and fibrosis in systemic sclerosis.


Systemic sclerosis (SSc) is an autoimmune disease mainly characterized by persistent inflammation and fibrosis. The receptor tyrosine kinase (RTK) signal pathway plays an important role in the process of SSc, and Grb2-associated binding protein (GAB) is crucial in activating RTK signalling. A previous study found elevated levels of GAB1 in bleomycin (BLM)-induced fibrotic lungs, but the effects of GAB1 in SSc remain unclear. Our aim was to investigate whether GAB1 was dysregulated and its potential role in SSc. Compared with healthy donors, we found GAB1 expression was 1.6-fold higher in peripheral blood mononuclear cells (PBMC), 2.5-fold higher in CD4 + T cells, and 2-fold higher in skin from of SSc patients (P < .01). At the same time, the levels of type one collagen (COLI) were also significantly increased (1.8-fold higher) in SSc skin. Additionally, BLM-induced SSc mice showed mRNA levels of Gab1 2-fold higher than saline-treated controls, and Gab1 expression correlated positively with collagen content. A further in vitro study showed silencing of GAB1 suppressed inflammatory gene expression in TNF-α induced fibroblasts. Additionally, GAB1 deficiency prominently inhibited cell proliferation and reduced COLI protein levels in TGF-β induced fibroblasts. Taken together, these data suggest that GAB1 has a relatively high expression rate in SSc, and knockdown of GAB1 may attenuate SSc by stimulating inflammatory and fibrotic processes.
Authors: Shi Xiangguang , Liu Qingmei , Zhao Han , Lu Jiaying , Huang Yan , Ma Yanyun , Xia Jingjing , Liu Mengguo , Tu Wenzhen , Jin Li , Wang Jiucun , Zhao Yinhuan , Wu Wenyu ,

Reference:

  1. ncbi.nlm.nih.gov. [Last access //].

Related products :

Catalog number Product name Quantity
27-476 SMAD5 undergoes copy number gain and increased expression, rather than loss of expression, and therefore does not act as a tumor-suppressor gene in hepatocellular carcinoma. Up-regulated Smad5 mediate 0.1 mg
27-475 SMAD5 undergoes copy number gain and increased expression, rather than loss of expression, and therefore does not act as a tumor-suppressor gene in hepatocellular carcinoma. Up-regulated Smad5 mediate 0.05 mg
EIAAB31630 Homo sapiens,Human,PMS2L14,PMS2L2,PMS2P2,PMS4,Postmeiotic segregation increased 2-like protein 14,Postmeiotic segregation increased protein 4,Putative postmeiotic segregation increased 2 pseudogene 2,
EIAAB31632 Homo sapiens,Human,PMS2L5,PMS2P5,PMS4,PMS7,Postmeiotic segregation increased 2-like protein 5,Postmeiotic segregation increased protein 4,Postmeiotic segregation increased protein 7,Putative postmeiot
AP-1131 Gene Expression: Mouse Inflammation cDNA Plate Array 4