GENTAUR Belgium BVBA BE0473327336 Voortstraat 49, 1910 Kampenhout BELGIUM Tel 0032 16 58 90 45
GENTAUR U.S.A Genprice Inc,Logistics 547 Yurok Circle, SanJose, CA 95123
Tel (408) 780-0908, Fax (408) 780-0908, [email protected]

Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, Gentaur another in time delivery

Pubmed ID: 31505422
Publication Date: 2019/09/07

Fangxiao Formula alleviates airway inflammation and remodeling in rats with asthma via suppression of transforming growth factor-β/Smad3 signaling pathway.

Asthma is a common obstructive airway disease characterized by inflammation and remodeling with a progressive decline in lung function. Fangxiao Formula (FXF) is an herbal medicine that has achieved significant clinical benefits toward asthma patients, but the relevant mechanism has not yet been clarified. The aim of this study was to determine the inhibitory effects of FXF on airway inflammation and remodeling, and investigate the activities of TGF‑β/Smads signaling pathway in the rat asthma model. Rats were sensitized by ovalbumin (OVA) for six weeks to establish the asthma experimental model. OVA-challenged animals were randomly divided into 5 groups and received different concentrations of FXF or dexamethasone. The animals in blank control group received saline only. Lung tissues were collected and analyzed for determining the inflammatory cells infiltration, HE and PAS staining, airway wall thickness and collagen deposition. The productions of inflammatory cytokine productions were analyzed by ELISA in the bronchoalveolar lavage (BAL) fluid. Immunohistochemical analysis was performed to measure the expression of α-SMA and PCNA in lung tissue after the treatment of FXF. The levels of TGF-β were assessed by both immunohistology and western blotting, and the expression of p-Smad2/3 proteins were determined by western blotting analysis. Our results indicated that FXF attenuated the infiltration of inflammatory cells, decreased the production of Th2 cytokines and simultaneously increased the levels of Th1 cytokine in the asthma rat model. In addition, FXF reduced allergen-induced increased airway wall thickness, goblet cell hyperplasia and collagen deposition. Furthermore, the expression levels of TGF-β and p-Smad3 were obviously reduced after the treatment of FXF. These results indicate that FXF alleviates airway inflammation and remodeling by restoring the balance of Th1/Th2 cytokines and the TGF-β/Smad-3 pathway, therefor providing potential therapeutic approach for asthmatic patients.
Authors: Ge Yuqing , Cheng Rubin , Sun Siya , Zhang Saijun , Li Lan , Jiang Jianping , Yang Chenxi , Xuan Xiaobo , Chen Jian ,


  1. [Last access 2019/09/07].

Related products :

Catalog number Product name Quantity
60511 SRE Reporter Kit (MAPK_ERK Signaling Pathway ),The MAPK_ERK signaling pathway is a major participant in the regulation of cell growth and differentiation. It can be activated by various extracellular 500 reactions
AA0028 IGF-1R Signaling Phospho-Specific Array includes 245 highly specific and well-characterized phosphorylation antibodies in the insulin-like growth factor 1 receptor signaling pathway. 2 Pieces/Box
30-618 SHC1 is a signaling adapter that couples activated growth factor receptors to signaling pathway. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to R 0.05 mg
86724 Transforming Growth Factor ß1 Peptide, TGF _ ß1 (60 _ 66),amide Formula C45H78N12O10 Sequence Lys_Val_Leu_Ala_Leu_Tyr_Asn_Lys_NH2 5mg
gen9363 SMAD1_HUMAN Transforming growth factor-beta-signaling protein 1 ELISA tesk kit 1