GENTAUR Belgium BVBA BE0473327336 Voortstraat 49, 1910 Kampenhout BELGIUM Tel 0032 16 58 90 45
GENTAUR U.S.A Genprice Inc,Logistics 547 Yurok Circle, SanJose, CA 95123
Tel (408) 780-0908, Fax (408) 780-0908, [email protected]

Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, Gentaur another in time delivery

Pubmed ID: 33938255
Publication Date: 2021/05/02

Regulation of Th1/Th2 and Th17/Treg by pDC/mDC imbalance in primary immune thrombocytopenia.


This study investigates the regulatory effect of plasmacytoid dendritic cells (pDC)/myeloid dendritic cells (mDC) imbalance on balance of Th1/Th2 and Th17/Treg in primary immune thrombocytopenia (ITP). A total of 30 untreated ITP patients and 20 healthy controls were recruited. Compared with healthy control, the pDC proportion of ITP patients was significantly reduced ( = 0.004), while the mDC proportion was not significantly changed ( = 0.681), resulting in a decrease in the pDC/mDC ratio ( = 0.001). Additionally, compared with controls, serum levels of interleukin (IL)-6, IL-12, and IL-23 were increased in ITP patients ( < 0.001), and mRNA levels of IL-12p40, IL-12p35, and IL-23p19 were also increased ( =0.014,  = 0.043,  < 0.001). Compared with the healthy control, the proportion of Th1 and Th17 cells in ITP patients increased ( = 0.001,  = 0.031). Serum levels of interferon gamma (IFN-γ) and IL-17 in ITP patients also increased ( = 0.025,  = 0.005). Furthermore, T-bet and RORγt mRNA levels were increased in peripheral blood of ITP patients ( = 0.018,  < 0.001). Correspondingly, the proportion of Th2 and Treg cells decreased ( = 0.007,  < 0.001), along with a decrease in serum IL-4 and transforming growth factor beta (TGF-β) ( = 0.028,  = 0.042), and an increase in GATA-3 mRNA ( < 0.001). However, there was no significant difference in Foxp3 mRNA levels ( = 0.587). Pearson correlation analysis showed that the proportion of total dendritic cells (DCs) was positively correlated with IL-12 ( = 0.526,  = 0.003) and IL-23 (= 0.501,  = 0.005) in ITP patients. Th1/Th2 ratio, IFN-γ, and IL-12 levels were negatively correlated with platelet counts ( = -0.494,  = 0.009;  = -0.415,  = 0.028;  = -0.492,  = 0.032). However, IL-23 was positively correlated with IL-17 (= 0.489,  = 0.006) and negatively correlated with platelet count ( = -0.564,  = 0.001). The ratio of IL-6 and Th17 cells was negatively correlated with platelet count ( = -0.443,  = 0.014;  = -0.471,  = 0.011). The imbalance of pDC/mDC and the increase of IL-6, IL-12, and IL-23 lead to the increased differentiation of CD4+ T cells into Th1 and Th17 cells, which might be the important mechanisms underlying the imbalance of Th1/Th2 and Th17/Treg in ITP patients.
Authors: Li Qinzhi , Liu Yang , Wang Xiujuan , Sun Mingling , Wang Lei , Wang Xinyou , Liu Ying , Fan Wenxia , Zhang Kaile , Sui Xiao , Guo Xinhong ,

Reference:

  1. ncbi.nlm.nih.gov. [Last access 2021/05/02].

Related products :

Catalog number Product name Quantity
25-105 ZFAT1 may be involved in transcriptional regulation. Overexpression of ZFAT1causes down-regulation of a number of genes involved in the immune response. Some genes are also up-regulated. 0.05 mg
QAM-TH17-1-SW Q-Analyzer Software for QAM-TH17-1 Antibody Array 1 module
QAH-TH17-1-SW Q-Analyzer Software for QAH-TH17-1 Antibody Array 1 module
S02-AAH-TH17-G1 Analysis Tool for AAH-TH17-G1 Antibody Array 1 module
S02-AAH-TH17-1 Analysis Tool for AAH-TH17-1 Antibody Array 1 module