GENTAUR Belgium BVBA BE0473327336 Voortstraat 49, 1910 Kampenhout BELGIUM Tel 0032 16 58 90 45
GENTAUR U.S.A Genprice Inc,Logistics 547 Yurok Circle, SanJose, CA 95123
Tel (408) 780-0908, Fax (408) 780-0908, [email protected]

Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, Gentaur another in time delivery

Pubmed ID: 35027540
Publication Date: 2022/01/13

Linking DNA repair and cell cycle progression through serine ADP-ribosylation of histones.


Although serine ADP-ribosylation (Ser-ADPr) by Poly(ADP-ribose)-polymerases is a cornerstone of the DNA damage response, how this regulates DNA repair and genome stability is unknown. Here, we exploit the ability to manipulate histone genes in Dictyostelium to identify that ADPr of the histone variant H3b at S10 and S28 maintains genome stability by integrating double strand break (DSB) repair with mitotic entry. Given the critical requirement for mitotic H3S10/28 phosphorylation, we develop separation of function mutations that maintain S10 phosphorylation whilst disrupting ADPr. Mechanistically, this reveals a requirement for H3bS10/28 ADPr in non-homologous end-joining by recruiting Ku to DSBs. Moreover, this also identifies H3bS10/S28 ADPr is critical to prevent premature mitotic entry with unresolved DNA damage, thus maintaining genome stability. Together, these data demonstrate how serine ADPr of histones coordinates DNA repair with cell cycle progression to maintain genome stability.
Authors: Brustel Julien , Muramoto Tetsuya , Fumimoto Kazuki , Ellins Jessica , Pears Catherine J , Lakin Nicholas D ,

Reference:

  1. ncbi.nlm.nih.gov. [Last access 2022/01/13].

Related products :

Catalog number Product name Quantity
29-802 Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation_deacetylation alters chromosome structure and affects transcription f 0.1 mg
27-349 Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation_deacetylation alters chromosome structure and affects transcription f 0.05 mg
28-136 Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation_deacetylation alters chromosome structure and affects transcription f 0.05 mg
EIAAB06190 CCP8,CCPG1,Cell cycle progression protein 1,Cell cycle progression restoration protein 8,CPR8,Homo sapiens,Human,KIAA1254
27-305 In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. CHEK2 is a cell cycle checkpoint regulator and putative tumor 0.05 mg