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Pubmed ID: 35027641
Publication Date: 2022/01/13

Demonstration of targeted crossovers in hybrid maize using CRISPR technology.


Naturally occurring chromosomal crossovers (CO) during meiosis are a key driver of genetic diversity. The ability to target CO at specific allelic loci in hybrid plants would provide an advantage to the plant breeding process by facilitating trait introgression, and potentially increasing the rate of genetic gain. We present the first demonstration of targeted CO in hybrid maize utilizing the CRISPR Cas12a system. Our experiments showed that stable and heritable targeted CO can be produced in F1 somatic cells using Cas12a at a significantly higher rate than the natural CO in the same interval. Molecular characterization of the recombinant plants demonstrated that the targeted CO were driven by the non-homologous end joining (NHEJ) or HDR repair pathways, presumably during the mitotic cell cycle. These results are a step towards the use of RNA-guided nuclease technology to simplify the creation of targeted genome combinations in progeny and accelerate breeding.
Authors: Kouranov Andrei , Armstrong Charles , Shrawat Ashok , Sidorov Vladimir , Huesgen Scott , Lemke Bryce , Boyle Timothy , Gasper Michelle , Lawrence Richard , Yang Samuel ,

Reference:

  1. ncbi.nlm.nih.gov. [Last access 2022/01/13].

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