GENTAUR Belgium BVBA BE0473327336 Voortstraat 49, 1910 Kampenhout BELGIUM Tel 0032 16 58 90 45
GENTAUR U.S.A Genprice Inc,Logistics 547 Yurok Circle, SanJose, CA 95123
Tel (408) 780-0908, Fax (408) 780-0908, [email protected]

Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, Gentaur another in time delivery

Pubmed ID: 35036061
Publication Date: 2021/12/14

A comparison of DNA repair pathways to achieve a site-specific gene modification of the Bruton's tyrosine kinase gene.


Gene editing utilizing homology-directed repair has advanced significantly for many monogenic diseases of the hematopoietic system in recent years but has also been hindered by decreases between and gene integration rates. Homology-directed repair occurs primarily in the S/G phases of the cell cycle, whereas long-term engrafting hematopoietic stem cells are typically quiescent. Alternative methods for a targeted integration have been proposed including homology-independent targeted integration and precise integration into target chromosome, which utilize non-homologous end joining and microhomology-mediated end joining, respectively. Non-homologous end joining occurs throughout the cell cycle, while microhomology-mediated end joining occurs predominantly in the S phase. We compared these pathways for the integration of a corrective DNA cassette at the Bruton's tyrosine kinase gene for the treatment of X-linked agammaglobulinemia. Homology-directed repair generated the most integration in K562 cells; however, synchronizing cells into G resulted in the highest integration rates with homology-independent targeted integration. Only homology-directed repair produced seamless junctions, making it optimal for targets where insertions and deletions are impermissible. Bulk CD34+ cells were best edited by homology-directed repair and precise integration into the target chromosome, while sorted hematopoietic stem cells contained similar integration rates using all corrective donors.
Authors: Gray David H , Santos Jasmine , Keir Alexandra Grace , Villegas Isaac , Maddock Simon , Trope Edward C , Long Joseph D , Kuo Caroline Y ,

Reference:

  1. ncbi.nlm.nih.gov. [Last access 2021/12/14].

Related products :

Catalog number Product name Quantity
BTN1A1 BTK Gene Bruton agammaglobulinemia tyrosine kinase
ICA1L IBTK Gene inhibitor of Bruton agammaglobulinemia tyrosine kinase
IBTK_MOUSE ELISA Kit FOR Inhibitor of Bruton tyrosine kinase; organism: Mouse; gene name: Ibtk 96T
pXTN pXTN GENE TAL nuclease Site-specific genetic modification plasmid 10 ug
U1915m CLIA Agammaglobulinaemia tyrosine kinase,ATK,B-cell progenitor kinase,BPK,Bpk,Bruton tyrosine kinase,Btk,Kinase EMB,Mouse,Mus musculus,Tyrosine-protein kinase BTK 96T