GENTAUR Belgium BVBA BE0473327336 Voortstraat 49, 1910 Kampenhout BELGIUM Tel 0032 16 58 90 45
GENTAUR U.S.A Genprice Inc,Logistics 547 Yurok Circle, SanJose, CA 95123
Tel (408) 780-0908, Fax (408) 780-0908, [email protected]

Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, Gentaur another in time delivery

Pubmed ID: 35081133
Publication Date: 2022/01/26

Proline-specific aminopeptidase P prevents replication-associated genome instability.


Genotoxic stress during DNA replication constitutes a serious threat to genome integrity and causes human diseases. Defects at different steps of DNA metabolism are known to induce replication stress, but the contribution of other aspects of cellular metabolism is less understood. We show that aminopeptidase P (APP1), a metalloprotease involved in the catabolism of peptides containing proline residues near their N-terminus, prevents replication-associated genome instability. Functional analysis of C. elegans mutants lacking APP-1 demonstrates that germ cells display replication defects including reduced proliferation, cell cycle arrest, and accumulation of mitotic DSBs. Despite these defects, app-1 mutants are competent in repairing DSBs induced by gamma irradiation, as well as SPO-11-dependent DSBs that initiate meiotic recombination. Moreover, in the absence of SPO-11, spontaneous DSBs arising in app-1 mutants are repaired as inter-homologue crossover events during meiosis, confirming that APP-1 is not required for homologous recombination. Thus, APP-1 prevents replication stress without having an apparent role in DSB repair. Depletion of APP1 (XPNPEP1) also causes DSB accumulation in mitotically-proliferating human cells, suggesting that APP1's role in genome stability is evolutionarily conserved. Our findings uncover an unexpected role for APP1 in genome stability, suggesting functional connections between aminopeptidase-mediated protein catabolism and DNA replication.
Authors: Silva Nicola , Castellano-Pozo Maikel , Matsuzaki Kenichiro , Barroso Consuelo , Roman-Trufero Monica , Craig Hannah , Brooks Darren R , Isaac R Elwyn , Boulton Simon J , Martinez-Perez Enrique ,

Reference:

  1. ncbi.nlm.nih.gov. [Last access 2022/01/26].

Related products :

Catalog number Product name Quantity
E0536b ELISA Bos taurus,Bovine,Cytosol aminopeptidase,LAP3,LAP-3,Leucine aminopeptidase 3,Leucyl aminopeptidase,Peptidase S,Proline aminopeptidase,Prolyl aminopeptidase 96T
E0536m ELISA Cytosol aminopeptidase,Lap3,LAP-3,Lapep,Leucine aminopeptidase 3,Leucyl aminopeptidase,Mouse,Mus musculus,Proline aminopeptidase,Prolyl aminopeptidase 96T
U0536b CLIA Bos taurus,Bovine,Cytosol aminopeptidase,LAP3,LAP-3,Leucine aminopeptidase 3,Leucyl aminopeptidase,Peptidase S,Proline aminopeptidase,Prolyl aminopeptidase 96T
E0536b ELISA kit Bos taurus,Bovine,Cytosol aminopeptidase,LAP3,LAP-3,Leucine aminopeptidase 3,Leucyl aminopeptidase,Peptidase S,Proline aminopeptidase,Prolyl aminopeptidase 96T
U0536m CLIA Cytosol aminopeptidase,Lap3,LAP-3,Lapep,Leucine aminopeptidase 3,Leucyl aminopeptidase,Mouse,Mus musculus,Proline aminopeptidase,Prolyl aminopeptidase 96T