Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, Gentaur another in time delivery

Pubmed ID :11134527
Publication Date : //

RNA degradosomes exist in vivo in Escherichia coli as multicomponent complexes associated with the cytoplasmic membrane via the N-terminal region of ribonuclease E.


RNase E isolated from Escherichia coli is contained in a multicomponent "degradosome" complex with other proteins implicated in RNA decay. Earlier work has shown that the C-terminal region of RNase E is a scaffold for the binding of degradosome components and has identified specific RNase E segments necessary for its interaction with polynucleotide phosphorylase (PNPase), RhlB RNA helicase, and enolase. Here, we report electron microscopy studies that use immunogold labeling and freeze-fracture methods to show that degradosomes exist in vivo in E. coli as multicomponent structures that associate with the cytoplasmic membrane via the N-terminal region of RNase E. Whereas PNPase and enolase are present in E. coli in large excess relative to RNase E and therefore are detected in cells largely as molecules unlinked to the RNase E scaffold, immunogold labeling and biochemical analyses show that helicase is present in approximately equimolar amounts to RNase E at all cell growth stages. Our findings, which establish the existence and cellular location of RNase E-based degradosomes in vivo in E. coli, also suggest that RNA processing and decay may occur at specific sites within cells.

Authors : Liou G G , Jane W N , Cohen S N , Lin N S , Lin-Chao S ,

Related products :

Catalog number Product name Quantity
P93191Hu01 Ribonuclease A8 (RNASE8) Organism: Homo sapiens (Human) Source: Escherichia coli 10ug
P93192Hu01 Ribonuclease A6 (RNASE6) Organism: Homo sapiens (Human) Source: Escherichia coli 50ug
P87642Hu01 Ribonuclease A12 (RNASE12) Organism: Homo sapiens (Human) Source: Escherichia coli 50ug
P90113Hu01 Ribonuclease T2 (RNASET2) Organism: Homo sapiens (Human) Source: Escherichia coli 100ug
P93192Hu01 Ribonuclease A6 (RNASE6) Organism: Homo sapiens (Human) Source: Escherichia coli 100ug
P84746Hu01 Ribonuclease A10 (RNASE10) Organism: Homo sapiens (Human) Source: Escherichia coli 50ug
P84746Hu01 Ribonuclease A10 (RNASE10) Organism: Homo sapiens (Human) Source: Escherichia coli 100ug
P90297Hu01 Ribonuclease A (RNASE1) Organism: Homo sapiens (Human) Source: Escherichia coli 50ug
P93190Hu01 Ribonuclease A4 (RNASE4) Organism: Homo sapiens (Human) Source: Escherichia coli 100ug
P93193Hu01 Ribonuclease A7 (RNASE7) Organism: Homo sapiens (Human) Source: Escherichia coli 10ug
P87642Hu01 Ribonuclease A12 (RNASE12) Organism: Homo sapiens (Human) Source: Escherichia coli 100ug
P87642Hu01 Ribonuclease A12 (RNASE12) Organism: Homo sapiens (Human) Source: Escherichia coli 10ug
P90113Hu01 Ribonuclease T2 (RNASET2) Organism: Homo sapiens (Human) Source: Escherichia coli 10ug
P93190Hu01 Ribonuclease A4 (RNASE4) Organism: Homo sapiens (Human) Source: Escherichia coli 10ug
P93191Hu01 Ribonuclease A8 (RNASE8) Organism: Homo sapiens (Human) Source: Escherichia coli 50ug
P93193Hu01 Ribonuclease A7 (RNASE7) Organism: Homo sapiens (Human) Source: Escherichia coli 50ug
P90297Hu01 Ribonuclease A (RNASE1) Organism: Homo sapiens (Human) Source: Escherichia coli 100ug
P84746Hu01 Ribonuclease A10 (RNASE10) Organism: Homo sapiens (Human) Source: Escherichia coli 10ug
P93192Hu01 Ribonuclease A6 (RNASE6) Organism: Homo sapiens (Human) Source: Escherichia coli 10ug
P93190Hu01 Ribonuclease A4 (RNASE4) Organism: Homo sapiens (Human) Source: Escherichia coli 50ug
P90113Hu01 Ribonuclease T2 (RNASET2) Organism: Homo sapiens (Human) Source: Escherichia coli 50ug
P93193Hu01 Ribonuclease A7 (RNASE7) Organism: Homo sapiens (Human) Source: Escherichia coli 100ug
P93191Hu01 Ribonuclease A8 (RNASE8) Organism: Homo sapiens (Human) Source: Escherichia coli 100ug
P90297Hu01 Ribonuclease A (RNASE1) Organism: Homo sapiens (Human) Source: Escherichia coli 10ug
B1212 M-E. Coli Broth USE : For detection, differntiation and enumeration of Escherichia coli and coliform in water samples by membrane filtration technique. 5x500gm


https://antibody-antibodies.com/ | https://gentaur.com/ | https://gen-script.com/ | https://diagenox.com/ | https://clonagen.com/ | http://gentaursearch.com/ | http://gentaurpub.com/ | https://gentaur-online.com/ | http://anti-anti-pdf.com/ | http://gentaur-worldwide.com/

 

Gentaur Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
[email protected] | Gentaur | Gentaur





Gentaur Ltd.
Unicorn House, Station Cl
Hertfordshire, Potters Bar EN6 1TL
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
[email protected] | Gentaur | Gentaur

 

 




Gentaur France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017
RIB 30004 00187 00010092253 10
BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG
IBAN FR76 3000 4001 8700 0100 9225 310
[email protected] | Gentaur | Gentaur

Gentaur GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: +49 0241 40 08 90 86, +49 0241 95 78 94 78, +49 0241 40 08 90 86
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
[email protected] | Gentaur | Gentaur

Gentaur U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
[email protected]

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




Gentaur Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
[email protected] | Gentaur | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






Gentaur Spain
tel:0911876558
[email protected] | Gentaur | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: [email protected] | Gentaur | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

Gentaur Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

[email protected] | Gentaur | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

Gentaur Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
[email protected] | Gentaur | Gentaur