Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, Gentaur another in time delivery

Pubmed ID :15292242
Publication Date : //

Formation of D-tyrosyl-tRNATyr accounts for the toxicity of D-tyrosine toward Escherichia coli.


D-Tyr-tRNATyr deacylase cleaves the ester bond between a tRNA molecule and a D-amino acid. In Escherichia coli, inactivation of the gene (dtd) encoding this deacylase increases the toxicity of several D-amino acids including D-tyrosine, D-tryptophan, and D-aspartic acid. Here, we demonstrate that, in a Deltadtd cell grown in the presence of 2.4 mm D-tyrosine, approximately 40% of the total tRNATyr pool is converted into D-Tyr-tRNATyr. No D-Tyr-tRNATyr is observed in dtd+ cells. In addition, we observe that overproduction of tRNATyr, tRNATrp, or tRNAAsp protects a Deltadtd mutant strain against the toxic effect of D-tyrosine, D-tryptophan, or D-aspartic acid, respectively. In the case of D-tyrosine, we show that the protection is accounted for by an increase in the concentration of L-Tyr-tRNATyr proportional to that of overproduced tRNATyr. Altogether, these results indicate that, by accumulating in vivo, high amounts of D-Tyr-tRNATyr cause a starvation for L-Tyr-tRNATyr. The deacylase prevents the starvation by hydrolyzing D-Tyr-tRNATyr. Overproduction of tRNATyr also relieves the starvation by increasing the amount of cellular L-Tyr-tRNATyr available for translation.

Authors : Soutourina Olga , Soutourina Julie , Blanquet Sylvain , Plateau Pierre ,

Related products :

Catalog number Product name Quantity
P91438Ra01 Tyrosine Hydroxylase (TH) Organism: Rattus norvegicus (Rat) Source: Escherichia coli 10ug
P91438Ra01 Tyrosine Hydroxylase (TH) Organism: Rattus norvegicus (Rat) Source: Escherichia coli 50ug
P91438Ra01 Tyrosine Hydroxylase (TH) Organism: Rattus norvegicus (Rat) Source: Escherichia coli 100ug
P91030Mu02 Protein Tyrosine Phosphatase Receptor Type C (PTPRC) Organism: Mus musculus (Mouse) Source: Escherichia coli 50ug
P91030Mu02 Protein Tyrosine Phosphatase Receptor Type C (PTPRC) Organism: Mus musculus (Mouse) Source: Escherichia coli 100ug
P91030Mu01 Protein Tyrosine Phosphatase Receptor Type C (PTPRC) Organism: Mus musculus (Mouse) Source: Escherichia coli 50ug
P91030Mu02 Protein Tyrosine Phosphatase Receptor Type C (PTPRC) Organism: Mus musculus (Mouse) Source: Escherichia coli 10ug
P91030Mu01 Protein Tyrosine Phosphatase Receptor Type C (PTPRC) Organism: Mus musculus (Mouse) Source: Escherichia coli 10ug
P91030Mu01 Protein Tyrosine Phosphatase Receptor Type C (PTPRC) Organism: Mus musculus (Mouse) Source: Escherichia coli 100ug
P91030Hu01 Protein Tyrosine Phosphatase Receptor Type C (PTPRC) Organism: Homo sapiens (Human) Source: Escherichia coli 100ug
P91030Hu01 Protein Tyrosine Phosphatase Receptor Type C (PTPRC) Organism: Homo sapiens (Human) Source: Escherichia coli 10ug
P91030Hu01 Protein Tyrosine Phosphatase Receptor Type C (PTPRC) Organism: Homo sapiens (Human) Source: Escherichia coli 50ug
P98809Hu01 Tyrosine 3_Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz) Organism: Homo sapiens (Human) Source: Escherichia coli 50ug
P98809Hu01 Tyrosine 3_Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz) Organism: Homo sapiens (Human) Source: Escherichia coli 10ug
P98809Hu01 Tyrosine 3_Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz) Organism: Homo sapiens (Human) Source: Escherichia coli 100ug
SL100497 Transfection Reagents and Kits, HapiCell™ Transfection Toxicity Removal Cocktail was our proprietary formulation for effectively eliminating DNA or siRNA transfection mediated toxicity. 2,5 ml
SL100497 Transfection Reagents and Kits, HapiCell™ Transfection Toxicity Removal Cocktail was our proprietary formulation for effectively eliminating DNA or siRNA transfection mediated toxicity. 1 ml
SL100496 Transfection Reagents and Kits, HapiCell™ Transfection Toxicity Removal Cocktail was our proprietary formulation for effectively eliminating DNA or siRNA transfection mediated toxicity. 125 ml
SL100497 Transfection Reagents and Kits, HapiCell™ Transfection Toxicity Removal Cocktail was our proprietary formulation for effectively eliminating DNA or siRNA transfection mediated toxicity. 0,1 ml
SL100496 Transfection Reagents and Kits, HapiCell™ Transfection Toxicity Removal Cocktail was our proprietary formulation for effectively eliminating DNA or siRNA transfection mediated toxicity. 50 ml
SL100496 Transfection Reagents and Kits, HapiCell™ Transfection Toxicity Removal Cocktail was our proprietary formulation for effectively eliminating DNA or siRNA transfection mediated toxicity. 5 ml
P71655Ge01 Escherichia coli Protein (ECP) Organism: General Source: Escherichia coli 100ug
P71655Ge01 Escherichia coli Protein (ECP) Organism: General Source: Escherichia coli 10ug
P71655Ge01 Escherichia coli Protein (ECP) Organism: General Source: Escherichia coli 50ug
SCH-4329-5004 MOUSE ANTI ESCHERICHIA COLI J5 LPS, Product Type Monoclonal Antibody, Specificity ESCHERICHIA COLI J5 LPS, Target Species Bacterial, Host Mouse, Format Purified, Isotypes IgG2b, Applications E, 0.1 mg


https://antibody-antibodies.com/ | https://gentaur.com/ | https://gen-script.com/ | https://diagenox.com/ | https://clonagen.com/ | http://gentaursearch.com/ | http://gentaurpub.com/ | https://gentaur-online.com/ | http://anti-anti-pdf.com/ | http://gentaur-worldwide.com/

 

Gentaur Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
[email protected] | Gentaur | Gentaur





Gentaur Ltd.
Unicorn House, Station Cl
Hertfordshire, Potters Bar EN6 1TL
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
[email protected] | Gentaur | Gentaur

 

 




Gentaur France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017
RIB 30004 00187 00010092253 10
BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG
IBAN FR76 3000 4001 8700 0100 9225 310
[email protected] | Gentaur | Gentaur

Gentaur GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: +49 0241 40 08 90 86, +49 0241 95 78 94 78, +49 0241 40 08 90 86
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
[email protected] | Gentaur | Gentaur

Gentaur U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
[email protected]

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




Gentaur Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
[email protected] | Gentaur | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






Gentaur Spain
tel:0911876558
[email protected] | Gentaur | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: [email protected] | Gentaur | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

Gentaur Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

[email protected] | Gentaur | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

Gentaur Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
[email protected] | Gentaur | Gentaur